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Time- and dose rate-related effects of internal 177Lu exposure on gene expression in mouse kidney tissue

ABSTRACT: Transcriptomic profiling of normal mouse kidney cortex and medulla following 177Lu irradiation

ORGANISM(S): Mus musculus

PROVIDER: GSE53164 | GEO | 2014/12/17

SECONDARY ACCESSION(S): PRJNA231030

REPOSITORIES: GEO

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Time- and dose rate-related effects of internal 177Lu exposure on gene expression in mouse kidney tissue

Project description:Transcriptomic profiling of normal mouse kidney cortex and medulla following 177Lu irradiation Total RNA was isolated from fresh-frozen tissue samples

2014-12-17 | E-GEOD-53164 | biostudies-arrayexpress

Transcriptional response of kidney tissue after 177Lu-octreotate administration in mice

Project description:Transcriptomic profiling of normal mouse kidney cortex and medulla following 177Lu irradiation

2014-03-05 | GSE44762 | GEO

Transcriptional response of kidney tissue after 177Lu-octreotate administration in mice

Project description:Transcriptomic profiling of normal mouse kidney cortex and medulla following 177Lu irradiation Total RNA was isolated from fresh-frozen tissue samples

2014-03-05 | E-GEOD-44762 | biostudies-arrayexpress

Biomarkers for radiation-induced renal toxicity following 177Lu-octreotate administration in mice

Project description:Transcriptomic profiling of normal mouse tissue and blood following 177Lu irradiation

2015-08-31 | GSE54674 | GEO

Biomarkers for radiation-induced renal toxicity following 177Lu-octreotate administration in mice

Project description:Transcriptomic profiling of normal mouse tissue and blood following 177Lu irradiation Total RNA was isolated from fresh-frozen tissue samples

2015-08-31 | E-GEOD-54674 | biostudies-arrayexpress

Transcriptomic profiling of human GOT1 and GOT2 tumor xenografts in Balb/c nude mice following 177Lu irradiation and/or Sonidegib treatment

Project description:The radiolabelled somatostatin analogue 177Lu-octreotate is a promising treatment option for malignant neuroendocrine tumors that overexpress somatostatin receptors. The human small intestine neuroendocrine tumor cell line GOT1 and Medullary thyroid carcinoma model GOT2 have shown promising treatment response to 177Lu-octreotate in xenografted mice. In clinical studies, however, only low cure rates have been achieved to date. In vitro and preclinical in vivo studies have shown that irradiation can up-regulate the expression of somatostatin receptors and thereby give an increased uptake of 177Lu-octreotate. The cellular processes that underlie positive treatment response to 177Lu-octreotate are otherwise largely unknown. Genome-wide analysis of tumor cell responses in this successful mouse model offers a venue to identify critical treatment parameters and to optimize clinical effectiveness of 177Lu-octreotate therapy. Combining 177Lu-octreotate with other anti-tumor agents has also been proposed as a strategy for optimization. Some studies have shown synergistic effects in tumor cell killing and volume reduction The hedgehog signaling pathway is involved in embryonic development and tissue regeneration and can be/is abnormally activated in various cancers. Inhibition of the hedgehog signaling pathway has yielded promising therapeutic effects on NE tumors and may potentially enhance the effects of 177Lu-octreotate treatment in patients.

2017-03-08 | GSE80022 | GEO

Real-time quantitative PCR analysis of mice kidney after administration of Lu-177-octreotate, Lu-177-octreotate + A1M and A1M

Project description:This work aimed to examine the regulation of apoptosis-related genes in kidney cortex and kindey medulla one and seven days after administration of 177Lu-octreotate with and without A1M and of A1M alone. C57BL/6N mice were injected with either 177Lu-octreotate + PBS, A1M + PBS or 177Lu-octreotate + A1M. Also, a control group was sham-treated with saline. Half of the animals in each treatment and control group were terminated by cardiac puncture one-day post-injection (1 dpi), and the remaining animals were terminated at 7dpi. Kidneys were dissected at the time of termination, snap-frozen in liquid nitrogen and stored at -80°C.

2024-04-01 | GSE200619 | GEO

Regulation of gene expression in GOT1 midgut carcinoid in nude mice following injection with 177Lu-octreotate

2017-12-31 | GSE80021 | GEO

Anti-CD37 radioimmunotherapy with 177Lu-NNV003 synergizes with the PARP inhibitor olaparib in treatment of non-Hodgkin’s lymphoma in vitro

Project description:Background and purpose: PARP inhibitors have been shown to increase the efficacy of radiotherapy in preclinical models. Radioimmunotherapy results in selective radiation cytotoxicity of targeted tumour cells. Here we investigate the combined effect of anti-CD37 β-emitting 177Lu-NNV003 radioimmunotherapy and the PARP inhibitor olaparib, and gene expression profiles in CD37 positive non-Hodgkin’s lymphoma cell lines. Materials and methods: The combined effect of 177Lu-NNV003 and olaparib was studied in seven cell lines using a fixed-ratio ray design, and combination index was calculated for each combination concentration. mRNA was extracted before and after treatment with the drug combination. After RNA-sequencing, hierarchical clustering was performed on basal gene expression profiles and on differentially expressed genes after combination treatment from baseline. Functional gene annotation analysis of significant differentially expressed genes after combination treatment was performed to identify enriched biological processes. Results: The combination of olaparib and 177Lu-NNV003 was synergistic in four of seven cell lines, antagonistic in one and both synergistic and antagonistic in two, depending on the concentration ratio between olaparib and 177Lu-NNV003. Cells treated with the combination significantly overexpressed genes in the TP53 signalling pathway. However, cluster analysis did not identify gene clusters that correlate with the sensitivity of cells to single agent or combination treatment. Conclusion: The cytotoxic effect of the combination of the PARP inhibitor olaparib and the β-emitting radioimmunoconjugate 177Lu-NNV003 was synergistic in the majority of tested lymphoma cell lines.

2022-04-15 | GSE178922 | GEO

B16-huCD20 tumors treated with 177Lu-TRT were subjected to multiplex analysis to study the tumor immune signature.

Project description:Targeted gene expression on 9 B16-huCD20 Melanoma tumor samples treated with 177Lu-radiolabed sdAbs using the murine panCancer Immune panel (NanoString Technologies)

2022-04-02 | GSE199624 | GEO

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