Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Kinetic analysis of carolacton-regulated genes

ABSTRACT: Transcriptional profiling of carolacton-treated Streptococcus mutans biofilm cells in comparison to untreated biofilms in a time series approach.

ORGANISM(S): Streptococcus mutans UA159

PROVIDER: GSE53264 | GEO | 2014/02/13

SECONDARY ACCESSION(S): PRJNA231476

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Kinetic analysis of carolacton-regulated genes

Project description:Transcriptional profiling of carolacton-treated Streptococcus mutans biofilm cells in comparison to untreated biofilms in a time series approach. Two-condition times series experiment, carolacton treated vs. untreated for timepoints 5, 20, 40, 60, 80, 100, 120, 160, 200, 240 and 300 minutes post-carolacton addition, 2 technical replicates of each sample (dye swap).

2014-02-13 | E-GEOD-53264 | biostudies-arrayexpress

Kinetic analysis of carolacton regulated genes

Project description:Transcriptional profiling of carolacton treated S. mutans biofilm cells in comparison to untreated biofilms in a time series approach.

2011-08-01 | GSE26441 | GEO

Kinetic analysis of carolacton regulated genes

Project description:Transcriptional profiling of carolacton treated S. mutans biofilm cells in comparison to untreated biofilms in a time series approach. Two condition times series experiment, carolacton treated vs. untreated for timepoints 20, 40, 60, 120 and 300 minutes post carolacton addition, 2 technical replicates of each sample (dye swap), conditions 20, 60, 300 Min in 3 biological replicates, condition 120 Min in 2 biological replicates, condition 40 min one biological sample

2011-07-31 | E-GEOD-26441 | biostudies-arrayexpress

Analysis of carolacton regulated genes

Project description:Transcriptional profiling of carolacton treated S. mutans biofilm cells in comparison to untreated biofilms after 8h of growth.

2011-08-01 | GSE26274 | GEO

Analysis of carolacton regulated genes

Project description:Transcriptional profiling of carolacton treated S. mutans biofilm cells in comparison to untreated biofilms after 8h of growth. Two condition experiment, carolacton treated vs. untreated, biological replicates: 4, 2 technical replicates of each sample (Dye Swap)

2011-07-31 | E-GEOD-26274 | biostudies-arrayexpress

Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton

Project description:Carolacton is a novel biofilm inhibitor that kills biofilm cells of Streptococcus mutans and inhibits growth of the clinically relevant and human pathogenic bacterium Streptococcus pneumoniae TIGR4 in nanomolar concentrations. Interestingly, Carolacton also inhibits Escherichia coli with a defective outer membrane protein TolC . The cellular target of Carolacton is still unknown. Here, we adressed the differential transcription of cellular RNAs when E.coli TolC was grown in the presence of Carolacton. This was done to identify transcriptional regulatory networks that are directly affected by treatment of the strain with Carolacton. In order to gain insights into the primary transcriptional response, early time-points were chosen for sampling, which should not reflect secondary responses (e.g. due to differences in growth phase, nutrient depletion etc.).

2017-02-15 | GSE93125 | GEO

Differential transcription of small RNAs and mRNAs of Streptococcus pneumoniae TIGR4 when grown with the biofilm inhibitor Carolacton

Project description:Carolacton is a novel biofilm inhibitor that kills biofilm cells of Streptococcus mutans in nanomolar concentrations. Interestingly, Carolacton also inhibits growth of the clinically relevant and human pathogenic bacterium Streptococcus pneumoniae TIGR4. The cellular target of Carolacton is still unknown. Here, we adressed the differential transcription of cellular RNAs when S. pneumoniae TIGR4 was grown in the presence of Carolacton. This was done to identify transcriptional regulatory networks that are directly affected by treatment of the pneumococcus with Carolacton. In order to gain insights into the primary transcriptional response, early time-points were chosen for sampling, which should not reflect secondary responses (e.g. due to differences in growth phase, drop in pH etc.). To achieve a thorough overview over all affected cellular RNA species, such as mRNAs, small regulatory RNAs and tRNAs, and not to lose small transcripts during library preparation, RNAs were separated according to size and used to construct two separate libraries for sequencing.

2016-09-19 | GSE76979 | GEO

Transcriptome analysis of Streptococcus mutans biofilms in response to starch and sucrose.

Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose at distinct stages of biofilm development.

2010-05-15 | GSE21831 | GEO

Transcription profiling of Streptococcus mutans grown in a biofilm with or without the presence of Veillonella parvula

Project description:Streptococcus mutans was grown for 48 h in a biofilm in the absence (single species) and in the presence (dual species) of Veillonella parvula. In addition V. parvula single species 48 h biofilms were grown, to be used as a control. RNA was harvested from all types of biofilms and the transcript levels of the two types of biofilms containing S. mutans were compared with the use of S. mutans microarrays. V. parvula RNA was hybridized to S. mutans microarrays as a control for possible cross-hybridisation.

2007-06-14 | E-MEXP-1034 | biostudies-arrayexpress

Transcriptome analysis of Streptococcus mutans biofilms in response to starch and sucrose.

Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose at distinct stages of biofilm development. RNA was extracted and purified from four replicate samples of each biofilm sample of interest and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cells grown to mid-log.

2010-05-15 | E-GEOD-21831 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data