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Gene expression of non-typeable Haemophilus influenzae strain R2866 and R2866Δ0112 mutant grown in supplemented brain-heart infusion medium for 7 hours

ABSTRACT: Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 7 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(7):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012.

ORGANISM(S): Haemophilus influenzae R2866 Haemophilus influenzae

PROVIDER: GSE53289 | GEO | 2013/12/13

SECONDARY ACCESSION(S): PRJNA231578

REPOSITORIES: GEO

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Gene expression of non-typeable Haemophilus influenzae strain R2866 and R2866Δ0112 mutant grown in supplemented brain-heart infusion medium for 4 hours

Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 4 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(4):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012.

2013-12-13 | GSE53287 | GEO

Gene expression of non-typeable Haemophilus influenzae strain R2866 and R2866Δ0112 mutant grown in supplemented brain-heart infusion medium for 4 hours

2013-12-13 | E-GEOD-53287 | biostudies-arrayexpress

Gene expression of non-typeable Haemophilus influenzae strain R2866 and R2866Δ0112 mutant grown in supplemented brain-heart infusion medium for 7 hours

Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 7 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(7):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012. R2866 wild-type (n=4) and R2866Δ0112 mutant (n=4) were grown for 7 hours in supplemented brain-heart infusrion medium The 4hr and 7hr analyses were performed separately and, therefore, the background corrections are different between the two experiments.

2013-12-13 | E-GEOD-53289 | biostudies-arrayexpress

Phasevarion of non-typeable Haemophilus influenzae R2866

2015-09-24 | GSE73349 | GEO

Phasevarion of non-typeable Haemophilus influenzae R2866

Project description:H. influenzae R2866 contains a phase-variable DNA methylase modA10, by virtue of 16 tetrameric repeats present after the start codon. Gain or loss of repeats during replication by slipped-strand mispairing can result in a non-functional reading frame. Microarray analyses of wild-type and insertionally inactivated modA were performed to determine genes affected by phase variable modification. Three replicate cultures were grown in either supplemented brain heart infusion broth (R2866 and R3763) or chemically defined media (R3790 and R3791) to an optical density at 600nm of 0.3 to 0.4 (mid-log phase). RNA was isolated by the method of Jahn et al.

2015-09-24 | E-GEOD-73349 | biostudies-arrayexpress

Pseudomonas aeruginosa PA14 differential gene expression in bqsR (PA14_29730) mutants

Project description:Before and after anaerobic Fe(II) shocked WT and ∆bqsR of late stationary phase P. aeruginosa PA14 strains Associated publication: Kreamer NN, Costa F, Newman DK. 2015. The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance. mBio 6(1):e02549-14. doi:10.1128/mBio.02549-14.

2015-02-01 | GSE65393 | GEO

Diel gene expression analysis of Trichodesmium erythraeum IMS101 in light and dark cycle

Project description:Investigation of whole genome gene expression level changes in Trichodesmium erythraeum IMS101 during a 24-hr light and dark cycle. The diel gene expression analyzed in this study is further described in Muñoz-Marin, M., I. N. Shilova, T. Shi, H. Farnelid & J. P. Zehr. 2019. The transcriptional cycle is suited to daytime N2 fixation in the unicellular cyanobacterium “Candidatus Atelocyanobacterium thalassa” (UCYN-A). mBio 10:e02495-18. https://doi.org/10.1128/mBio.02495-18.

2017-06-11 | GSE99896 | GEO

Haemophilus influenzae R2866

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Haemophilus influenzae R2866

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Expression profiling of Moraxella catarrhalis RH4 during in vitro growth in brain heart infusion (BHI) medium

Project description:Transcriptome analysis of M. catarrhalis RH4 predicted coding sequences and clustered regularly interspaced short palindromic repeats (CRISPRs) regions. The results described in this study are further discussed in De Vries, S.P.W., van Hijum, S.A.F.T., Schueler, W., Riesbeck, K., Hays, J.P., Hermans, P.W.M., Bootsma, H.J.; Genome analysis of Moraxella catarrhalis strain RH4: a human respiratory tract pathogen; Journal of Bacteriology

2010-05-07 | GSE21632 | GEO

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