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Effect of TetR family regulator SAOUHSC_02897 deletion in S. aureus NCTC8325

ABSTRACT: To analyze function of TetR family regulator SAOUHSC_02897, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target SAOUHSC_02897 was replaced by ermB gene.

ORGANISM(S): Staphylococcus aureus subsp. aureus NCTC 8325 Staphylococcus aureus

PROVIDER: GSE53432 | GEO | 2013/12/18

SECONDARY ACCESSION(S): PRJNA231982

REPOSITORIES: GEO

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Effect of TetR family regulator SAOUHSC_02897 deletion in S. aureus NCTC8325

Project description:To analyze function of TetR family regulator SAOUHSC_02897, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target SAOUHSC_02897 was replaced by ermB gene. Bacteria grown in the wells of biofilm formation at 12 hours, and the RNA level of wild type NCTC8325 and SAOUHSC-02897 mutant were compared.

2013-12-18 | E-GEOD-53432 | biostudies-arrayexpress

Effect of different Two-component systems system deletion in S. aureus NCTC8325

Project description:To analyze function of three Two-component systems SAOUHSC_00184-185, SAOUHSC_01313-1314, and SAOUHSC_01980-1981, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target TCSs were replaced by ermB gene.

2013-09-30 | GSE51197 | GEO

Effect of SAOUHSC_02810 deletion in S. aureus NCTC8325

2014-04-07 | GSE56283 | GEO

Effect of different Two-component systems system deletion in S. aureus NCTC8325

2013-09-30 | E-GEOD-51197 | biostudies-arrayexpress

Effect of SAOUHSC_02810 deletion in S. aureus NCTC8325

Project description:To analyze function of SAOUHSC_02810, we have employed whole genome microarray expression profiling to identify genes transcription changed in mutant strain. SAOUHSC_02810 were replaced by ermB gene. Cells were grown to OD600=2.0, and the RNA level of wild type NCTC8325 and SAOUHSC_02810 mutant were compared.

2014-04-07 | E-GEOD-56283 | biostudies-arrayexpress

A TetR-family regulator of an RND efflux system that directs artemisinin resistance in Vibrio cholerae

Project description:Artemisinin (ARS) displayed bactericidal activity against Vibrio cholerae. To assess the mechanistic details of its antibacterial action, we have isolated V. cholerae mutants with enhanced ARS resistance and identified a gene (VCA0767), whose loss-of-function resulted in the ARS resistance phenotypes. This gene (atrR) encodes a TetR family transcriptional regulator, and its deletion mutant displayed the reduction in ARS-induced ROS formation and DNA damage. Transcriptomic analysis revealed that the genes encoding an RND efflux pump operon (vexRAB) and the outer membrane component (tolC) were highly upregulated in the artR mutant, suggesting that AtrR might act as a negative regulator of this operon and tolC. Gene deletion of vexR, vexB or tolC abrogated the ARS resistance of the atrR mutant and, more importantly, the ectopic expression of VexAB-TolC was sufficient for the ARS resistance, indicating that the increased expression of the VexAB-TolC efflux system is necessary and sufficient for the ARS resistance of the atrR mutant. The cytoplasmic accumulation of ARS was compromised in the vexBtolC mutant, suggesting that the VexAB-TolC might be the primary efflux system exporting ARS to reduce its toxicity inside of the bacterial cells. The atrR mutant displayed resistance to erythromycin as well in a VexR-dependent manner. This result suggests that AtrR may act as a global regulator responsible for preventing intracellular accumulation of toxic chemicals by enhancing the RND efflux system.

2023-10-16 | GSE245142 | GEO

Functional characterization of TetR-like transcriptional regulator PA3973 from Pseudomonas aeruginosa [RNA-seq]

Project description:Pseudomonas aeruginosa, a human opportunistic pathogen, is a common cause of nosocomial infections. Its ability to survive under different conditions relies on a complex regulatory network engaging transcriptional regulators controlling metabolic pathways and capabilities to efficiently use the available resources. P. aeruginosa PA3973 encodes a putative TetR family transcriptional regulator, with a helix-turn-helix motif involved in DNA binding. We applied transcriptome profiling (RNA-seq), and genome-wide identification of binding sites using ChIP-seq to unravel the biological role of PA3973.

2022-11-22 | GSE211771 | GEO

TetR Family Regulator brpT Modulates Biofilm Formation in Streptococcus sanguinis.

Project description: Not available

| S-EPMC5207742 | biostudies-literature

Functional characterization of TetR-like transcriptional regulator PA3973 from Pseudomonas aeruginosa [ChIP-seq]

Project description:Pseudomonas aeruginosa PA3973 encodes a putative TetR family transcriptional regulator, with a helix-turn-helix motif involved in DNA binding. We applied phenotype analyses, as well as transcriptome profiling (RNA-seq), and genome-wide identification of binding sites using ChIP-seq to unravel the biological role of PA3973. The ChIP-seq analysis identified more than 300 PA3973 binding sites in the P. aeruginosa genome, among them 139 were located in intergenic regions. The 13 bp sequence was identified as the preferential binding site of PA3973. The PA3973 regulon encompasses genes involved in stress response, including the putative PA3973-PA3970 operon. Transcriptional profiling of P. aeruginosa PAO1161 overexpressing PA3973 showed changes in the mRNA level of 648 genes; among them, 374 were down-regulated. Concomitantly, ChIP-seq analysis identified more than 300 PA3973 binding sites in the P. aeruginosa genome, among them 139 were located in intergenic regions. The 13 bp sequence was identified as the preferential binding site of PA3973.

2022-11-22 | GSE211769 | GEO

A novel TetR-like transcriptional regulator is induced in acid-nitrosative stress and controls expression of an efflux pump in mycobacteria

Project description:Mycobacterium tuberculosis has the ability to survive inside macrophages under acid-nitrosative stress. M. tuberculosis Rv1685c and its orthologue in M. smegmatis, MSMEG_3765, are induced on exposure to acid-nitrosative stress. Both genes are annotated as TetR transcriptional regulators, a family of proteins that regulate a wide range of cellular activities, including multidrug resistance, carbon catabolism and virulence. Here we demonstrate that MSMEG_3765 is co-transcribed with the upstream genes MSMEG_3762 and MSMEG_3763, encoding efflux pump components. RTq-PCR and GFP-reporter assays showed that the MSMEG_3762/63/65 gene cluster, and the orthologous region in M. tuberculosis (Rv1687c/86c/85c), was up-regulated in a MSMEG_3765 null mutant, suggesting that MSMEG_3765 acts as a repressor, typical of this family of regulators. We further defined the MSMEG_3765 regulon using genome-wide transcriptional profiling and confirmed that the MSMEG_3762/63/65 promoter is induced under acid-nitrosative stress. A putative 36 bp regulatory motif was identified upstream of the gene clusters in both M. smegmatis and M. tuberculosis and purified recombinant MSMEG_3765 protein was found to bind to DNA fragments containing this motif from both M. smegmatis and M. tuberculosis upstream regulatory regions. These results suggest that the TetR repressor MSMEG_3765/Rv1685c controls expression of an efflux pump with an, as yet, undefined role in the mycobacterial response to acid-nitrosative stress.

2017-10-24 | E-MTAB-5869 | biostudies-arrayexpress

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