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Detection of juvenile hormone-like function of diofenolan in Daphnia magna using in vivo short-term screening assay, TG211 Annex 7 reproduction test and microarray analysis

ABSTRACT: Juvenile hormone (JH) and JH agonists have been reported to induce male offspring production in various daphnids including Daphnia magna. We have recently established a short-term in vivo screening method to detect chemicals having JH activity, induction of male offspring, using adult D. magna. Diofenolan has been developed to be a JH agonist in insects, but no male induction in daphniids has been studied. In this study, we found the male offspring induction by diofenolan with a short-term in vivo screening method, and the most potent male offspring production activity among JH analogs studied using the OECD TG211 Annex 7. Transcriptome analysis of diofenolan-exposed D. magna showed up-regulation of JH-responsive genes. These results clearly demonstrated that diofenolan has JH activities in D. magna using the short-term in vivo screening assay, OECD TG211 Annex 7 and microarray analysis.

ORGANISM(S): Daphnia magna

PROVIDER: GSE53570 | GEO | 2014/12/01

SECONDARY ACCESSION(S): PRJNA232398

REPOSITORIES: GEO

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Comparative ovarian microarray analysis of juvenile hormone-responsive genes in water flea Daphnia magna

Project description:The freshwater zooplankton Daphnia magna has been used for the validation of chemical toxicity tests such as OECD Test Guideline 202 and 211. Previously, it has been demonstrated that treatment of juvenile hormones (JHs) or their analogs to mother daphnids could induce male offspring production. Based on this funding, a rapid screening method for detection of chemical with JH-activity was recently developed by using adult D. magna. This screening system can validate whether arbitrary chemical has JH-activity by investigating the male offspring inducibility. Although this short-term screening system will be possible to enhance a throughput of detection efficiency, JH-responsive genes in the ovary including developing oocytes at JH-sensitive period for male sex determination are still largely undescribed. Here, we conducted comparative microarray analyses using ovary treated by fenoxycarb (Fx; artificial JH agonist) and methyl farnesoate (MF; a putative innate JH in daphnids) to make the comprehensive gene catalogs in response to JH molecules in ovary including developing oocytes at JH-sensitive period for male sex determination. We successfully created the comprehensive ovarian gene catalogs in response to Fx and MF. Furthermore, we demonstrated that the hemoglobin genes are well-conserved JH-responsive element even in the ovary, and a potential toxic effect of JH agonist is suppression of vitellogenin gene expression that might cause reduction of offspring number. This is first report demonstrating the differential transcriptomics profiles between MF and artificial JH agonist in D. magna ovary, making it possible for understanding the tissue-specific mode-of-action of JH molecule.

2017-05-01 | GSE81083 | GEO

Molecular impact of juvenile hormone agonists on neonatal Daphnia magna

Project description:Daphnia magna has been used extensively to evaluate organism- and population-level responses to pollutants in acute toxicity and reproductive toxicity tests. We have previously reported that exposure to juvenile hormone (JH) agonists results in a reduction of reproductive function and production of male offspring in a cyclic parthenogenesis, D. magna. Recent advances in molecular techniques have provided tools to better understand the responses to pollutants in aquatic organisms including D. magna. DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to JH agonists; methoprene (125, 250 and 500 ppb), fenoxycarb (0.5, 1 and 2 ppb), and epofenonane (50, 100 and 200 ppb). Exposure to these JH analogs resulted in chemical specific patterns of gene expression. The heat map analyses based on hierarchical clustering revealed a similar pattern between treatments with a high dose of methoprene and with epofenonane. In contrast, treatment with low to middle doses of methoprene resulted in similar profiles to fenoxycarb treatments. Hemoglobin and JH epoxide hydrolase genes clustered to be JH-responsive genes. These data suggest that fenoxycarb has high activity as a JH agonist, methoprene shows high toxicity and epofenonane works through a different mechanism compared with other JH analogs, agreeing with data of previously reported toxicity tests. In conclusion, D. magna DNA microarray is useful for classification of JH analogs and identification of JH-responsive genes. Juvenile hormone agonists induced gene expression in daphnids neonates was measured at 48 hours after exposure to methoprene (125, 250, 500 ppb), fenoxycarb (0.5, 1, 2 ppb), epofenonane (50, 100, 200) and DMF as a control. Three independent experiments were performed at each chemicals.

2013-12-31 | E-GEOD-47990 | biostudies-arrayexpress

Molecular impact of juvenile hormone agonists on neonatal Daphnia magna

2013-12-31 | GSE47990 | GEO

Gene expression profiles in liver of F344 rat treated with chemical

2013-04-02 | GSE45650 | GEO

Gene expression profiles in liver of F344 rat treated with chemical

Project description:The rodent carcinogenicity test requires extremely long test term and large examination cost. Therefore, now we need to develop a short term and low cost screening method. We already developed a chemical carcinogenicity short term screening method CARCINOscreenM-BM-.. This study was performed to validate this screening method. We conducted 28 days-repeated dose experiments in male F344 rats with 4 carcinogens and 2 non-carcinogens, and the gene expression profiles in liver were measureed by custom oligo microarrays. Two-condition experiment, control vs. chemical treated rat liver. Biological replicates: 4 control, 4 treated, control samples were pooled. One replicate per array. Compound treatments: 4 carcinogens: Hexachloroethane, 1,2,3-Trichloropropane, 1-Amino-2-methylanthraquinone, 3-(4-Chlorophenyl)-1,1-dimethylurea 2 non-carcinogens: 1,2-Dichlorobenzene and Tetracycline hydrochloride

2013-04-02 | E-GEOD-45650 | biostudies-arrayexpress

Identification of metabolic pathways in Daphnia magna exposed to selective serotonin reuptake inhibitors using transcriptomic, immunocytochemistry and physiological responses.

Project description:Assessing the risks of long-term exposure to low doses of pharmaceuticals is an identified research need, particularly for those that may act as neural disruptors in non-vertebrate species. Selective serotonin reuptake inhibitors (SSRIs) act by blocking the re-uptake of serotonin in the nerve synapses, increasing the effective concentration of serotonin in the intra-synaptic space and therefore stimulating serotoninergic neurons. This effect is used worldwide to treat clinical depression in humans, with the consequence of their widespread release into the environment. SSRIs have been found to alter the reproductive physiology of D. magna and other invertebrates in a biphasic way. Low levels of fluoxetine stimulated offspring production in Daphnia magna and Ceriodaphnia dubia at 36 and 50 Âµg/l, respectively, but higher exposure levels inhibited reproduction in the same species. In this study we explore the hypothesis that SSRI can affect D. magna juvenile developmental rates and offspring production disrupting serotonin activity that is known to regulate carbohydrate and oxidative metabolic pathways. The primary mode of action was tested using transcriptomic analyses of juveniles and adults exposed to SSRIs (fluoxetine 80 ppbs, fluovoxamine 30 ppbs) and serotonin-immunocytochemistry assays of D. magna brains. Reproduction, respirometry and biochemical measurements allowed to related gene and immunological base effects to phenotypic responses.

2013-03-15 | E-GEOD-45053 | biostudies-arrayexpress

Whole organism transcriptomic analysis provides mechanistic insight into the acute toxicity of emamectin benzoate in Daphnia magna

Project description:This experiment was conducted to study the short-term (12h) transcriptional responses in Daphnia magna after exposure to the anti-sea lice chemical emamectin benzoate (EMB). The microarray results were further vefiried using qPCR. The gene exression responses were linked to adverse effects after 48h exposure, in order to supply knowledge for environmental hazard assessment of this chemical in non-target crustaceans. Neonatal (<24h) Daphnia magna were exposed to 7.8-2000 pM waterborne emamectin benzoate for 12h. Microarray analysis was performed using pooled whole-organism D. magna (8 individuals) and 4 biological replicates were analyzed for each treatment group.

2016-06-30 | E-GEOD-83859 | biostudies-arrayexpress

Transcription profiling of juvenile Daphnia manga following exposure to the pesticides methomyl and propanil

Project description:Juvenile daphnia magna (<24hrs old, n=25 / test vessel) were exposed to methomyl (M) a carbamate insecticide (10.52ug/l), propanil (P), an analide herbicide (363ug/l), an independent action (IA) mixture of M (10.52ug/l and P (363ug/l) representing effect concentration 1 (EC1) for each chemical, based on standard immobility assay (OECD) or to control (untreated medium). All exposures were carried out in reconstituted water (see protocol GROWTH). Treated vessels were spiked with stock solutions of pesticides dissolved directly into reconstituted water. Test vessels were 1L glass beakers arranged in a randomised block design. Transcriptomic screening was used to assess the mechanism of toxicity of the two single pesticides and to explain (in part) the interaction of methomyl and propanil in an independent action (IA) EC1 mixture.

2010-09-15 | E-TABM-793 | biostudies-arrayexpress

Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex

Project description:Under favorable conditions, the micro-crustacean Daphnia pulex produces female offspring by parthenogenesis, whereas in response to various unfavorable external cues, they produce male offspring to induce sexual reproduction (environmental sex determination: ESD). We recently established a suitable experimental system for ESD studies using D. pulex WTN6 strain, in which the sex of the offspring can be regulated simply by alterations in day-length; long-day and short-day conditions can induce female and male offspring respectively. Taking advantage of this system, we have already demonstrated that methyl farnesoate (MF) synthesis is necessary for male offspring production. Further, we identified ionotropic glutamate receptors as an upstream regulator of MF signaling by transcriptome analysis. Despite these findings, the molecular mechanisms associated with MF signaling have not yet been well elucidated in D. pulex. To more comprehensively understand the molecular mechanisms of ESD, we analyzed the whole metabolic profiles of mother daphniids reared under long-day (female-producing) and short-day (male-producing) conditions using mass spectrometry based metabolomics. We discovered that pantothenate (vitamin B5), a known precursor to coenzyme A, was significantly accumulated in response to the short-day condition. To confirm the role of pantothenate in D. pulex ESD, this metabolite was administered to mother daphniids (under long-day conditions) resulting in a significantly increased proportion of male offspring producing mothers. This study not only provides several candidates for further investigations into the molecular changes involved in male offspring production, but also novel insights into the metabolic mechanisms of the ESD system in D. pulex.

2017-04-03 | MTBLS259 | MetaboLights

Long-term exposure of Daphnia magna to polystyrene microplastics induces changes in their proteome.

Project description:In the past years, the research focus on the effects of microplastics (MP) on aquatic organisms extended from marine systems towards freshwater systems. An important freshwater model organism in the MP field is the cladoceran Daphnia, which plays a central role in lacustrine ecosystems and has been established as a test organism in ecotoxicology. To investigate the effects of MP on Daphnia magna, we performed a chronic exposure experiment with polystyrene MP under strictly standardized conditions. Chronic exposure of D. magna to PS microparticles led to a significant reduction in body length and number of offspring. To shed light on underlying molecular mechanisms induced by microplastic ingestion in D. magna, we assessed the effects of PS-MP at the proteomic level.

2021-07-07 | PXD025108 | Pride

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