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Micromolar fluoride alters ameloblast lineage cells in vitro

ABSTRACT: Previously characterized ameloblast-lineage cells were used in this study to determine whether micromolar levels of fluoride could be directly affect these cells. Keywords: substance response

ORGANISM(S): Homo sapiens

PROVIDER: GSE5365 | GEO | 2007/07/20

SECONDARY ACCESSION(S): PRJNA96261

REPOSITORIES: GEO

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Micromolar fluoride alters ameloblast lineage cells in vitro

Project description:Previously characterized ameloblast-lineage cells were used in this study to determine whether micromolar levels of fluoride could be directly affect these cells. Six samples altogether were analyzed in this study. Every two of them were from a same biological souce and subject to with or without the treatment of 10uM NaF (the only difference between the two samples.) The experiment was repeated 3 times.

2010-06-30 | E-GEOD-5365 | biostudies-arrayexpress

Homo sapiens

Project description:Micromolar fluoride alters ameloblast lineage cells in vitro

| PRJNA96261 | ENA

Fluoride alters Ca2+ signaling and mitochondrial function in enamel cells [Mouse]

Project description:We analyzed the effects of fluoride exposure in mouse enamel cells to assess its impact on cell Ca2+ signaling

2020-01-14 | GSE143535 | GEO

Fluoride alters Ca2+ signaling and mitochondrial function in enamel cells [Human]

Project description:We analyzed the effects of fluoride exposure in mouse enamel cells to assess its impact on cell Ca2+ signaling

2020-01-14 | GSE143534 | GEO

MEMO1 is required for ameloblast maturation and functional enamel formation

Project description:Coordinated mineralization of soft tissue is central to organismal form and function, while dysregulated mineralization underlies several human pathologies. Oral epithelial derived ameloblasts are polarized, secretory cells responsible for generating enamel, the most mineralized substance in the human body. Defects in ameloblast development result in enamel anomalies, including amelogenesis imperfecta. Identifying proteins critical in ameloblast development can provide insight into specific pathologies associated with enamel related disorders or more broadly, mechanisms of mineralization. Previous studies identified a role for MEMO1 in bone mineralization; however, whether MEMO1 functions in the generation of additional mineralized structures remains unknown. Here, we identify a critical role for MEMO1 in enamel mineralization. First, we identified that Memo1 is expressed in ameloblasts and that conditional deletion of Memo1 from ameloblasts results in enamel defects, characterized by a decline in mineral density and tooth integrity. Molecular profiling of ameloblasts and their progenitors in Memo1 oral epithelial mutants revealed a disruption to cytoskeletal associated genes and a reduction in late stage ameloblast markers, relative to controls. Histology revealed that the molecular defects correlated with a disruption of the apical Tome’s process and the basolateral interacting, papillary layer, in Memo1 mutant ameloblasts. Finally, deletion of Memo1 from an oral epithelial ameloblast cell line, followed by cellular and molecular analyses, revealed altered cytoskeletal networks, relative to control cells. Collectively, our findings integrate MEMO1 into an emerging network of molecules important for ameloblast development and provide both in vivo and in vitro systems to further interrogate the relationship of cytoskeletal and amelogenesis-related defects.

2023-08-03 | GSE235869 | GEO

Gene expression profiling of HOS (human osteosarcoma) cells exposed to fluoride

Project description:Though fluoride is considered an essential trace element, chronic exposure to fluoride is known to cause toxic effects. Chronic exposure of high concentration of fluoride may leads to fluorosis. To understand the molecular mechanism of fluoride induced toxicity gene expression profiling was performed on osteosarcoma cells (HOS). Cells were exposed to sub-lethal concentration of fluoride (8 ppm) for 30 days. Our result demonstrates that fluoride alters multiple biological pathways including bone development, osteoblast differentiation and apoptotic pathways.

2016-06-30 | GSE70719 | GEO

Global gene expression changes in osteoblasts from fluoride-tolerant mice

Project description:In order to define the underlying mechanism of fluoride resistance in mammals and provide a theoretical basis for fluorosis treatment, high-throughput sequencing was applied to map the genetic changes of fluoride-resistant mouse osteoblasts. Fluoride-tolerant MC3T3-E1 cells were developed by gradient fluoride exposure. The differentially expressed genes of fluorine-resistant MC3T3-E1 cells were identified by high-throughput sequencing. High-throughput RNA sequencing identified 2702 differentially expressed genes (DEGs) showed more than 2-fold difference in 30ppm FR MC3T3-E1 cells, of which 17 DEGs were associated with ferroptosis.

2022-05-25 | GSE203511 | GEO

Gene expression profiling of HOS (human osteosarcoma) cells exposed to fluoride

2016-06-30 | E-GEOD-70719 | biostudies-arrayexpress

Transcriptome of human odontoblast, pre-secretory ameloblast and secretory ameloblast

Project description:The goal of the study was to characterize the whole genome transcriptome profiles of human ameloblasts and odontoblasts, evaluate molecular pathways and advance our knowledge of the human tooth development. We found that during primary tooth formation, odontoblasts expressed 14,802 genes, presecretory ameloblasts 15,179 genes and secretory ameloblasts 14,526 genes. Four human fetuses were obtained at ages 15-20 weeks gestation, immediately placed on ice and the tooth buds dissected from the jaws, placed in RNAlater and refrigerated at 4C for 1-4 weeks to allow decalcification by EDTA. The tissue was then frozen and stored at -80C. The tissue was sectioned at -35C at a thickness of 7 microns. These sections were used for laser capture microdissection (LCM) to isolate the human odontoblasts and ameloblasts in different stages of enamel formation, using static image settings. In total, 4 odontoblast, 4 pre-secretory ameloblast and 4 secretory ameloblast pooled samples were used for RNA extraction and microarray analysis.

2014-11-14 | E-GEOD-63289 | biostudies-arrayexpress

The differentially expressed genes of A. ferrooxidans ATCC 23270 at different time after introduced fluoride

Project description:The deleterious fluoride ions, which released from fluoride in uranium during its bioleaching, strongly influenced microbial growth, energy intake, enzyme activity and relative metabolism. Whole-genome microarrays were used to obtain a comprehensive description of the molecular response by A. ferrooxidans ATCC 23270 at 4.8 mM fluoride stress.

2016-01-01 | GSE76450 | GEO

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