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Microarray analysis of the Asian seabass (Lates calcarifer) gonads

ABSTRACT: In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2–4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads.

ORGANISM(S): Lates calcarifer

PROVIDER: GSE53898 | GEO | 2015/01/08

SECONDARY ACCESSION(S): PRJNA233662

REPOSITORIES: GEO

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Microarray analysis of the Asian seabass (Lates calcarifer) gonads

Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2M-bM-^@M-^S4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads. Total 22 samples: Adult Ovaries (F3-stage; 5 years old fish) : 4 Adult Testes (M3-stage; 5 years old fish) : 4 Early Testes (M3-stage; 8-9 months old fish) : 3 Early Transforming Gonads (>2 years old fish) : 3 Late Transforming Gonads (>2 years old fish) : 4 Undifferentiated Gonads (4.5 months old fish) : 4

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Microarray analysis of the Asian seabass (Lates calcarifer) brains

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Project description:In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g Â± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. Our customised agilent microarray platform (GPL17855), were used to investigate the liver transcriptomic expression of the different Groups fed with different Feeds. No differential expression were detected between commerical Feeds (B, C, E and F). A total of 397 differentially expressed transcripts (Foldchange cutoff: -1.5â¤ or â¥1.5, Pvalue with FDR (<0.05) were detected between Feed D and control Feed A. Total: 24 samples (4 biological samples per Group; 6 Different Groups in total). Groups (A-F) were fed with Feeds (A-F) respectively for 61 days.

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