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Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

ABSTRACT: Local renin antiotensin systems have been identified for many extra-renal sites. Bone marrow has been proposed as one such site, although the nature of the renin-expressing cell type(s) has not been established. Affymetrix microarrays were used to characterize the expression profile of renin-expressing GFP positive cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE53916 | GEO | 2014/02/17

SECONDARY ACCESSION(S): PRJNA233958

REPOSITORIES: GEO

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Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

Project description:Local renin antiotensin systems have been identified for many extra-renal sites. Bone marrow has been proposed as one such site, although the nature of the renin-expressing cell type(s) has not been established. Affymetrix microarrays were used to characterize the expression profile of renin-expressing GFP positive cells. Green fluorescent protein positive cells were sorted from whole bone marrow collected from adult transgenic mice. Bone marrow from several mice was collected and pooled on the day of a FACS sort. A portion of this was reserved for RNA extraction while the remainder was sorted. RNA was prepared with Trizol. Bone marrow collection and sorting was performed 3 times so that microarrays could be run in triplicate.

2014-02-17 | E-GEOD-53916 | biostudies-arrayexpress

scRNAseq libraries of pooled kidney samples from wild type (ZF1) versus CRISPR-targeted renin knockout (ZF2) zebrafish

Project description:Libraries were made to compare the transcriptome of renin lineage cells (RLCs) from wild-type versus ren knockout zebrafish kidneys. RLCs were FAC sorted from pooled kidneys of ren+/+ or ren-/- zebrafish, which carried ren:RFP and acta2:EGFP reporter genes, allowing the isolation of renin-expressing cells and smooth muscle cells.

2022-06-10 | E-MTAB-11079 | biostudies-arrayexpress

Mouse kidney arteriolar smooth muscle cells derived from the renin cell lineage: control vs. forskolin treatment to induce renin expression

2012-02-25 | GSE31780 | GEO

Single-cell RNA-seq in Reninnull cells and WT renin cells

Project description:We have used single-cell RNA-seq to identify transcriptional differences between WT renin cells and renin cells with deletion of the renin gene

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Mouse kidney arteriolar smooth muscle cells derived from the renin cell lineage: control vs. forskolin treatment to induce renin expression

Project description:miRNA profiling of mouse kidney arteriolar smooth muscle cells (aSMCs) of the renin lineage comparing control untreated cells with cells treated with forskolin to induce renin expression. Two condition experiment: control untreated aSMCs vs forskolin treated aSMCs; Biological replicates: control 3, treated 3; independently grown and harvested. One replicate per array.

2012-02-25 | E-GEOD-31780 | biostudies-arrayexpress

Genome-wide maps of chromatin state in renin cells [ChIP-Seq]

Project description:We generated genome-wide chromatin-state maps of mouse renin cells: chronically recruited cells and As4.1 cell line. We found that H3K27ac can discriminate a set of regulatory regions unique for the renin cells.

2020-10-31 | GSE117722 | GEO

RNA-seq of Stimulated/Recruited Renin Cells

Project description:This experiment was performed to study the expression profile of renin cells from mice subjected to homeostatic threat (hypotension). It was also used to compare expression of proliferation associated genes and tumor suppressors with existing cell line data.

2020-03-05 | GSE146386 | GEO

Generation of a Recombinant Akabane Virus Expressing Enhanced Green Fluorescent Protein.

Project description: Not available

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Characterisation of cancer stem cells and the renin-angiotensin system in colon adenocarcinoma

Project description:LC-MS/MS Proteomics was performed to determine proteomic differences between colon adenocarcinoma (CA) tissues and patient-matched normal colon tissues, CA-derived primary cell lines and NC-derived primary cells, and between LGCA tissues and cell lines and HGCA tissues and cell lines. Biological processes which may link the renin-angiotensin system and CA were investigated, revealing enrichment of various signalling pathways that may play roles in CA onset and progression directly or via the renin-angiotensin system.

2022-08-12 | PXD024449 | Pride

Gene expression profiles of renin producing cells in the adult kidney (captopril treated animals) isolated from Renin-YFP transgenic mice using FACS (GUDMAP Series ID:46).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Renin-YFP transgenic mice were utilized to isolate renin producing cells from the kidneys of Adult mice treated with captopril, which was provided in the drinking water at a concentration of 0.5mg/ml. In addition, mice were given low-salt chow. Mice were treated for two weeks before renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

2012-12-05 | E-GEOD-42712 | biostudies-arrayexpress

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