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Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Dinoroseobacter shibae DFL 12 = DSM 16493

PROVIDER: GSE53967 | GEO | 2014/02/01

SECONDARY ACCESSION(S): PRJNA234048

REPOSITORIES: GEO

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Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-02-01 | E-GEOD-53967 | biostudies-arrayexpress

Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum [RNA-seq]

Project description:Transcriptome of D. shibae 5 h in the light and dark after 12 days in co-culture with the dinoflagellate P. minimum.

2014-02-01 | GSE53871 | GEO

Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum at different growth stages

Project description:Transcriptome of D. shibae 5 h in the light after 18,24 and 30 days in coculture with the dinoflagellate P. minimum

2014-05-01 | GSE55371 | GEO

Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum [RNA-seq]

Project description:Transcriptome of D. shibae 5 h in the light after 12 days in co-culture with the dinoflagellate P. minimum. Two biological replicates.

2014-02-01 | E-GEOD-53871 | biostudies-arrayexpress

Light-dark changes in the transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

Project description:Comparison of the transcriptome of D. shibae 5 h in the light versus 5 h in the dark after 12 days in co-culture with the dinoflagellate P. minimum.

2014-02-01 | GSE53846 | GEO

Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum at different growth stages

Project description:Transcriptome of D. shibae 5 h in the light after 18,24 and 30 days in coculture with the dinoflagellate P. minimum two biological replicates

2014-05-01 | E-GEOD-55371 | biostudies-arrayexpress

Light-dark changes in the transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

Project description:Comparison of the transcriptome of D. shibae 5 h in the light versus 5 h in the dark after 12 days in co-culture with the dinoflagellate P. minimum. Two biological replicates of each condition are compared.

2014-02-01 | E-GEOD-53846 | biostudies-arrayexpress

Identification of Genetic Modules Mediating the Jekyll and Hyde Interaction of Dinoroseobacter shibae with the Dinoflagellate Prorocentrum minimum.

Project description:The co-cultivation of the alphaproteobacterium Dinoroseobacter shibae with the dinoflagellate Prorocentrum minimum is characterized by a mutualistic phase followed by a pathogenic phase in which the bacterium kills aging algae. Thus it resembles the "Jekyll-and-Hyde" interaction that has been proposed for other algae and Roseobacter. Here, we identified key genetic components of this interaction. Analysis of the transcriptome of D. shibae in co-culture with P. minimum revealed growth phase dependent changes in the expression of quorum sensing, the CtrA phosphorelay, and flagella biosynthesis genes. Deletion of the histidine kinase gene cckA which is part of the CtrA phosphorelay or the flagella genes fliC or flgK resulted in complete lack of growth stimulation of P. minimum in co-culture with the D. shibae mutants. By contrast, pathogenicity was entirely dependent on one of the extrachromosomal elements of D. shibae, the 191 kb plasmid. The data show that flagella and the CtrA phosphorelay are required for establishing mutualism and prove a cell density dependent killing effect of D. shibae on P. minimum which is mediated by an unknown factor encoded on the 191 kb plasmid.

| S-EPMC4643747 | biostudies-literature

Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

Project description:Transcriptome of Dinoroseobacter shibae in co-culture with the dinoflagellate Prorocentrum minimum

| PRJNA234048 | ENA

The Influence of Genes on the "Killer Plasmid" of <i>Dinoroseobacter shibae</i> on Its Symbiosis With the Dinoflagellate <i>Prorocentrum minimum</i>.

Project description:The marine bacterium Dinoroseobacter shibae shows a Jekyll-and-Hyde behavior in co-culture with the dinoflagellate Prorocentrum minimum: In the initial symbiotic phase it provides the essential vitamins B12 (cobalamin) and B1 (thiamine) to the algae. In the later pathogenic phase it kills the dinoflagellate. The killing phenotype is determined by the 191 kb plasmid and can be conjugated into other Roseobacters. From a transposon-library of D. shibae we retrieved 28 mutants whose insertion sites were located on the 191 kb plasmid. We co-cultivated each of them with P. minimum in L1 medium lacking vitamin B12. With 20 mutant strains no algal growth beyond the axenic control lacking B12 occurred. Several of these genes were predicted to encode proteins from the type IV secretion system (T4SS). They are apparently essential for establishing the symbiosis. With five transposon mutant strains, the initial symbiotic phase was intact but the later pathogenic phase was lost in co-culture. In three of them the insertion sites were located in an operon predicted to encode genes for biotin (B7) uptake. Both P. minimum and D. shibae are auxotrophic for biotin. We hypothesize that the bacterium depletes the medium from biotin resulting in apoptosis of the dinoflagellate.

| S-EPMC8831719 | biostudies-literature

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