Comparative total RNA and mRNA sequencing and systems analysis reveals nascent transcriptional response to early HIV-1 infection in a CD4+ T cell line

ABSTRACT: To systematically investigate early host transcriptional response to HIV-1 infection, including polyadenylated and non-polyadenylated transcripts, we separately sequenced Total RNAs (Total RNA-seq) from HIV-1-infected SupT1 cells, a human CD4+ T cell line. We show many non-polyadenylated RNAs were also differentially expressed upon HIV-1 infection, as expected only detected by Total RNA-seq. Interestingly, we identified 8 times more differentially expressed genes at 12 hour post-infection by Total RNA-seq than by mRNA-seq, mostly protein-coding genes.

OTHER RELATED OMICS DATASETS IN: PRJNA234297

ORGANISM(S): Homo sapiens Human immunodeficiency virus 1

PROVIDER: GSE53993 | GEO | 2014/06/10

SECONDARY ACCESSION(S): PRJNA234297

REPOSITORIES: GEO