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Human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

ABSTRACT: Transcriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE54146 | GEO | 2015/02/01

SECONDARY ACCESSION(S): PRJNA235303

REPOSITORIES: GEO

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human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

Project description:Transcriptional profiling of human hTERT-RPE1 cell spheroids comparing Control siRNA transfected hTERT-RPE1 cell spheroids with those transfected with YAP1 siRNA. Two-condition experiment, Control siRNA vs.YAP1 siRNA hTERT-RPE1 cell spheroids. Biological replicates: 1 Control siRNA, 1YAP1 siRNA transfected, independently grown and harvested. Bothreplicates per array.

2015-02-01 | E-GEOD-54146 | biostudies-arrayexpress

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Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion.

2014-03-01 | GSE44540 | GEO

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Project description:Gene expression was profiled in unperturbed RPE1-hTERT cells by RNA-seq.

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Gene expression in hTERT-RPE1 cells with overexpression of MFRP

Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion. RNA was prepared from hTERT-RPE1 cells overexpressing GFP-MFRP and control cells. Both conditions were done in triplicate. Affymetrix GeneChip Human Genome U133Plus2.0 arrays were used to interrogate changes in gene expression. Image data were quantified with Affymetrix Expression Console Software and normalized with Robust Multichip Analysis.

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Project description:RNA-seq upon MYCN activation in the hTERT-immortalized MYCN retinal pigmented epithelial cell line (RPE1–MYCN-ER). The parental hTERT-immortalized retinal pigment epithelium (hTERT-RPE1) was used as a control as this cell line does not carry the MYCN:ER expression construct. Both cell lines were treated with tamoxifen (400nM; 4-OHT) for MYCN induction (with 4-OHT or not). Analysis was performed 24 h, 48 h and 72 h upon MYCN activation, including four biological replicates per condition.

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Single-cell replication timing analysis of hTERT-RPE1 cells depleted for SAF-A protein

Project description:The impact of depleting SAF-A (HNRNPU) on the genome-wide replication timing program in human hTERT-RPE1 cells was assessed by a single-cell replication timing analysis.

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RNA-seq in human RPE1-hTERT cells

Project description:RNA-seq in human RPE1-hTERT cells

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ChIP-seq of old and new H3.3 in RPE1-hTERT cells

Project description:On of the H3.3 genes (H3F3B) was C-terminally tagged with a recombination-induced tag exchange (RITE) tag in human RPE1-hTERT cells to follow old (V5 tag) and new (FLAG tag) H3.3 and measure the dynamics of H3.3. New H3.3 was induced by activating Cre recombinase with 4-hydrotamoxifen (4OHT). ChIP-seq was done on old H3.3 and new H3.3 8h and 24h after 4OHT treatment.

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McGill EMC Community projects Release 7 for cell line "hTERT RPE1" - samples

Project description:Complete reference epigenome (as defined by IHEC) of normal hTERT RPE1 cell line as well as hTERT RPE1 cell lines engineered to express EPC1-PHF1 and JAZF1-SUZ12 fusion proteins, found in Endometrial Stromal Sarcoma.

| EGAD00001007680 | EGA

Homo sapiens

Project description:human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

| PRJNA235303 | ENA