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Mycobacterium tuberculosis: Control Vs db-cAMP treated cells

ABSTRACT: Transcriptional profiling of Mycobacterium tuberculosis CDC1551 comparing control butyric acid-treated cells with 20mM db-cAMP treated cells after 2hrs of treatment with shaking at 200rpm at 37C.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE54289 | GEO | 2014/01/23

SECONDARY ACCESSION(S): PRJNA236135

REPOSITORIES: GEO

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Mycobacterium tuberculosis: Control Vs db-cAMP treated cells

Project description:Transcriptional profiling of Mycobacterium tuberculosis CDC1551 comparing control butyric acid-treated cells with 20mM db-cAMP treated cells after 2hrs of treatment with shaking at 200rpm at 37C. Two-condition experiment, butyric acid (Control) Vs db-cAMP treated cells (Test). Biological replicates: 3 control, 3 test. One replicate per array.

2014-01-23 | E-GEOD-54289 | biostudies-arrayexpress

CD-1mice: db/db vs db/+

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diabetic nephropathy streptozotocin db db

Project description:diabetes, mouse models, diabetic nephropathy, streptozotocin db/db Keywords: other

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Profiling pancreatic islets from obese db/db and lean control db/+ mice

Project description:This program addresses the molecular basis of beta-cell failure associated with the development of type 2 diabetes in the db/db mice. Specifically, which genes are differentially expressed in pancreatic islets of the db/db mice compared to the control db/+ mice? The db/db mice islets profiling data was analyzed by identifying genes that were up- and down-regulated at selected p value and fold change in the islets of db/db mice compared to the corresponding db/+ controls.

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Project description:Purpose: RNA seq analysis were to compare and contrast the gene expression profile involved in the dedifferentiation of db/db islets in type 2 diabetes Methods: Islets of wild type, db/+ and db/db were purified using perfusion from 12 week old mice and RNA were isolated. Islated RNA were used in RNA seq to understand the expression pattern Results: Using an optimized data analysis workflow, we mapped about 10 million sequence reads per sample to the mouse genome (build mm9) and identified 16,014 transcripts WT, db/+ and db/db mice islets with TopHat workflow. Hierarchical clustering of differentially expressed genes uncovered there role in type 2 diabetes. Data analysis with TopHat workflows revealed a significant overlap yet provided complementary insights in transcriptome profiling. Conclusions: We characterised and identified genes involved in dedifferentiation of islets.

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Profiling epididymal white adipose tissue (eWAT) from obese db/db and lean control db/+ mice

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Gene expression changes in aortas of db/db versus control db/+ mice

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Profiling epididymal white adipose tissue (eWAT) from obese db/db and lean control db/+ mice

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2013-03-22 | E-GEOD-38082 | biostudies-arrayexpress

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