Transcriptomics,Genomics

Dataset Information

51

Induced CD103+ Dendritic Cells


ABSTRACT: Multiple subsets of FLT3L-dependent dendritic cells (DCs) control T cell tolerance and immunity. In mouse tissues, CD8α-like DCs are identified by CD103 expression. This DC subset efficiently enters lymph nodes and cross-presents antigens, rendering CD103+ DCs promising targets for therapeutic tolerance induction or vaccination. However, only limited numbers of CD103+ DCs can be isolated with current methods. Moreover, bone marrow cultures with FLT3L produce complex mixtures of DC subsets. We developed a novel method for generating large numbers of Batf3-dependent CD103+ DCs. We used microarray analysis to compare in vitro generated CD103+ and CD103- DCs and correlated their expression patterns to published profiles and signatures of DC subsets. Overall design: We generated iCD103 DCs according to our new method. We sorted CD11c+B220-CD103hi and CD11c+B220-CD103- DC subsets and processed these populations for RNA extraction and hybridization to Agilent microarrays.

INSTRUMENT(S): Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)

SUBMITTER: Robert Geffers  

PROVIDER: GSE54472 | GEO | 2014-12-31

SECONDARY ACCESSION(S): PRJNA236596

REPOSITORIES: GEO

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Publications

Selective and efficient generation of functional Batf3-dependent CD103+ dendritic cells from mouse bone marrow.

Mayer Christian Thomas CT   Ghorbani Peyman P   Nandan Amrita A   Dudek Markus M   Arnold-Schrauf Catharina C   Hesse Christina C   Berod Luciana L   Stüve Philipp P   Puttur Franz F   Merad Miriam M   Sparwasser Tim T  

Blood 20140806 20


Multiple subsets of FMS-like tyrosine kinase 3 ligand (FLT3L)-dependent dendritic cells (DCs) control T-cell tolerance and immunity. In mice, Batf3-dependent CD103(+) DCs efficiently enter lymph nodes and cross-present antigens, rendering this conserved DC subset a promising target for tolerance induction or vaccination. However, only limited numbers of CD103(+) DCs can be isolated with current methods. Established bone marrow culture protocols efficiently generate monocyte-derived DCs or produc  ...[more]

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