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Histone dosage and gene expression changes in Halobacterium salinarum

ABSTRACT: We set out to determine a) if histone in Halobacterium salinarum regulates transcription and b) whether the magnitude and extent of these changes matches those observed in organisms which use histone protein as their primary DNA packaging agent. To this end, gene expression data for a histone knock-out (Δura3ΔhpyA) strain versus parent (Δura3) were collected.

ORGANISM(S): Halobacterium salinarum NRC-1

PROVIDER: GSE54599 | GEO | 2015/08/31

SECONDARY ACCESSION(S): PRJNA237184

REPOSITORIES: GEO

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Histone dosage and gene expression changes in Halobacterium salinarum

Project description:We set out to determine a) if histone in Halobacterium salinarum regulates transcription and b) whether the magnitude and extent of these changes matches those observed in organisms which use histone protein as their primary DNA packaging agent. To this end, gene expression data for a histone knock-out (?ura3?hpyA) strain versus parent (?ura3) were collected. The histone deletion mutant and parent strain, at log and stationary phase, were compared to the common reference strain NRC-1 (log). There are three biological replicates each, plus dye-flips, for a total of 24 arrays

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