Polysomes from DENR knockdown cells

ABSTRACT: The aim was to identify transcripts that are poorly translated upon knockdown of DENR. Lysates from control (GFP) and DENR knockdown S2 cells were run on polysome gradients. RNA from the (80S peak + polysome peaks) was isolated and analyzed by microarrays. In parallel, total RNA from the cells was also profiled as a normalization control and profiled. The 'translation score' of translated mRNA to total RNA was then calculated for control and DENR knockdown cells.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE54625 | GEO | 2014/06/30

SECONDARY ACCESSION(S): PRJNA237227

REPOSITORIES: GEO