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Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity [BNC1]

ABSTRACT: TGF-b1-stimulation induces an epithelial dedifferentiation-process, throughout which epithelial cell sheets disintegrate and gradually switch into fibroblastic-appearing cells (EMT-like transition). Several transcription factors, some of them being TGF-b1-responsive, are functionally involved in such a switch and affect epithelial differentiation and plasticity. We used microarray-based gene expression profiling of mammary epithelial cells that actively undergo TGF-b1-induced epithelial dedifferentiation. Further, we determined gene expression changes in Basonuclin-1 knock-down cells in conjunction with TGF-b1-stimulation in order to determine a possible effect of Bnc1 on TGF-b1-responsive genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE54715 | GEO | 2014/03/01

SECONDARY ACCESSION(S): PRJNA237493

REPOSITORIES: GEO

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Basonuclin-1 modulates epithelial plasticity and TGF-?1-induced loss of epithelial cell integrity [BNC1]

Project description:TGF-b1-stimulation induces an epithelial dedifferentiation-process, throughout which epithelial cell sheets disintegrate and gradually switch into fibroblastic-appearing cells (EMT-like transition). Several transcription factors, some of them being TGF-b1-responsive, are functionally involved in such a switch and affect epithelial differentiation and plasticity. We used microarray-based gene expression profiling of mammary epithelial cells that actively undergo TGF-b1-induced epithelial dedifferentiation. Further, we determined gene expression changes in Basonuclin-1 knock-down cells in conjunction with TGF-b1-stimulation in order to determine a possible effect of Bnc1 on TGF-b1-responsive genes. Cells were transfected with non-silencing (control) siRNA or siRNA against Basonuclin-1 (Bnc1) for 24hrs. Subsequently, cells were treated with 5ng/ml TGF-b1 or left untreated for additional 24hrs. The experiments were performed as independent biological triplicate.

2014-03-01 | E-GEOD-54715 | biostudies-arrayexpress

Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity [NIAC-NTR]

Project description:TGF-b1-stimulation induces an epithelial dedifferentiation-process, throughout which epithelial cell sheets disintegrate and gradually switch into fibroblastic-appearing cells (EMT-like transition). The purpose of these profiles was to identify differentially expressed genes that are regulated transcriptionally. Standard microarry-based gene expression profiles measure steady-state RNA but do not provide insight into underlying regulatory principles. NIAC-NTR-based gene expression profiling (Kenzelmann et al., PNAS, 2007) essentially enables the dissection of transcriptionally versus non-transcriptionally regulated genes within respective analysed time-frames. Briefly, NIAC-NTR relies on incorporation of 4sU (thio-uridine) into nascent RNA, which can subsequently be specifically isolated by custom-made columns. Total- and enriched (4sU-labeled) are then further processed for microarray gene expression profiling by standard procedures. This dataset complements previously released data of NIAC-NTR-based gene expression profiling of cells treated with TGF-b1 and 4sU for 2hrs [GSE23833]. The present data and files represent the outcome of NIAC-NTR-based gene expression profiling of cells treated with TGF-b1 for 24hrs and incubation with 4sU for the last 2hrs (22hrs-24hrs of TGF-b1-stimulation). NIAC-NTR: Non Invasive Application and Capture of Newly Transcribed RNA

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Basonuclin-1 modulates epithelial plasticity and TGF-M-NM-21-induced loss of epithelial cell integrity [NIAC-NTR]

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2014-03-01 | E-GEOD-54716 | biostudies-arrayexpress

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Project description:Rattus norvegicus Bnc1, basonuclin 1 [Source:RGD Symbol;Acc:1309042], is expressed in 2 baseline experiment(s);

| ENSRNOG00000019770 | ExpressionAtlas

BNC1

Project description:Monodelphis domestica BNC1, basonuclin 1 [Source:NCBI gene;Acc:100011949], is expressed in 1 baseline experiment(s);

| ENSMODG00000019380 | ExpressionAtlas

BNC1

Project description:Sus scrofa BNC1, basonuclin 1 [Source:VGNC Symbol;Acc:VGNC:85851], is differentially expressed in 1 experiment(s);

| ENSSSCG00000001798 | ExpressionAtlas

BNC1

Project description:Sus scrofa BNC1, basonuclin 1 [Source:VGNC Symbol;Acc:VGNC:85851], is expressed in 1 baseline experiment(s);

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BNC1

Project description:Macaca mulatta BNC1, basonuclin 1 [Source:VGNC Symbol;Acc:VGNC:70270], is expressed in 1 baseline experiment(s);

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BNC1

Project description:Chlorocebus sabaeus BNC1, basonuclin 1 [Source:HGNC Symbol;Acc:HGNC:1081], is differentially expressed in 1 experiment(s);

| ENSCSAG00000017303 | ExpressionAtlas

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