Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides

ABSTRACT: A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1∆rnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3´-to-5´ exoribonucleases.

ORGANISM(S): Cereibacter sphaeroides 2.4.1

PROVIDER: GSE54750 | GEO | 2014/02/06

SECONDARY ACCESSION(S): PRJNA237547

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides

Project description:A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1M-bM-^HM-^Frnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3M-BM-4-to-5M-BM-4 exoribonucleases. Total RNA from exponentially grown cultures (OD660 0,4) of 2.4.1 wild type and 2.4.1M-bM-^HM-^Frnj has been isolated in triplicates using the hot phenol method and prepared for sequencing on a Illumina Genome Analyzer IIx machine.

2014-02-06 | E-GEOD-54750 | biostudies-arrayexpress

PpsR regulon in Rhodobacter sphaeroides

Project description:mRNA levels were measured in Rhodobacter sphaeroides 2.4.1 at 20% O2 and 0.5% O2, Rhodobacter sphaeroides 2.4.1 App11 (AppA-null), Rhodobacter sphaeroides 2.4.1 (pPNs) and PpsR mutant PPS2-4. The mRNA samples were prepared from cultures supplied with 20% O2, 1% CO2, and 79% N2, and grown in the dark to an OD of 0.18. The mRNA levels for each strain was measured three times. Keywords: repeat sample

2004-07-03 | GSE1515 | GEO

Rhodobacter sphaeroides WT 2.4.1 under singlet oxygen stress

Project description:Transcriptional and translational profiling of R. sphaeroides WT 2.4.1 under singlet oxygen stress compared to control (no stress) conditions

2012-11-14 | GSE42244 | GEO

Transcriptome response to nitrosative stress in Rhodobacter sphaeroides 2.4.1

2012-11-11 | GSE33641 | GEO

RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1

Project description:This SuperSeries is composed of the following subset Series: GSE39711: RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from chromatin immuno-precipitation GSE39712: RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from gene expression profiling Refer to individual Series

2012-08-02 | E-GEOD-39806 | biostudies-arrayexpress

Transcriptome analysis of the Rhodobacter sphaeroides RNase III mutant (inactivated enzyme) by total RNA sequencing

Project description:RNase III is an important and highly conserved endoribonuclease known to impact rRNA, mRNA and ncRNA abundances by RNA processing. In this study we analyzed the effects of an inactivation of RNase III (inactivated through substitution of two strictly conserved amino acids within the active enzyme center) on the transcriptome of the facultative phototrophic model organism Rhodobacter sphaeroides.

2024-02-19 | GSE236804 | GEO

Transcriptome response to nitrosative stress in Rhodobacter sphaeroides 2.4.1

Project description:DNA microarray analysis was employed to investigate the transcriptome response to nitrosative stress in a non-denitrifying facultative photosynthetic bacterium Rhodobacter sphaeroides 2.4.1. We focused on the role played by a nitric oxide-response transcriptional regulator NnrR in the response. The transcriptome profiles of R. sphaeroides 2.4.1 and its nnrR mutant before and after exposure to nitrosating agents S-nitrosoglutathione (GSNO) or sodium nitroprusside (SNP) under semiaerobic conditions were analyzed. R. sphaeroides 2.4.1 and its nnrR mutant were cultivated in glass bottles under semiaerobic growth conditions. When the optical density at 600 nm reached approximately 0.15-0.2, 1 mM GSNO or SNP was added to the medium. RNA was isolated from a 50 ml aliquot of the culture prior to the addition of GSNO or SNP and at 15 min after the addition. The experiment was performed in duplicate independent cultures.

2012-11-11 | E-GEOD-33641 | biostudies-arrayexpress

Transcriptome analysis and 5‘-end mapping of total RNA from Rhodobacter sphaeroides wild type and RNase E (rne) mutant strain, grown under aerobic, microaerobic or phototrophic conditions

Project description:The conserved endoribonuclease RNase E is essential in Rhodobacter sphaeroides and acts as global regulator of the transcriptome. By comparison of an RNase E mutant (showing reduced enzyme activity) with the Rhodobacter sphaeroides wild type, both grown under three different growth conditions, we analysed the impact of RNase E on the adaption of Rhodobacter sphaeroides to different growth conditions.

2022-12-28 | GSE200990 | GEO

R. sphaeroides Δlov vs. R. sphaeroides 2.4.1 (microarobic conditions)

Project description:Transcriptional profiling of R. sphaeroides Δlov comparing to control R. sphaeroides 2.4.1 under microarobic conditions

2011-10-26 | GSE33194 | GEO

Rhodobacter sphaeroides 2.4.1

Project description:Rhodobacter sphaeroides 2.4.1 genome sequencing

| PRJNA330068 | ENA