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Expression profiling of SMMC-7721 cells: stably express lncRNA-ATB vs pcDNA3.1

ABSTRACT: lncRNA-ATB induced transcriptional changes in SMMC-7721

ORGANISM(S): Homo sapiens

PROVIDER: GSE54798 | GEO | 2014/02/08

SECONDARY ACCESSION(S): PRJNA237664

REPOSITORIES: GEO

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Expression profiling of SMMC-7721 cells: stably express lncRNA-ATB vs pcDNA3.1

Project description:lncRNA-ATB induced transcriptional changes in SMMC-7721 Two-condition experiment, control (pcDNA3.1) vs lncRNA-ATB expression (pcDNA3.1-ATB). Each has 3 biological repeats.

2014-02-08 | E-GEOD-54798 | biostudies-arrayexpress

Expression profiling of SMMC-7721 cells: stably express lncRNA-PRAL vs control

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Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.

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Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

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Expression data from SMMC-7721 cells

Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells The liver cancer cell line SMMC-7721 transfected with either the control or POH1 siRNAs were subjected to a genome wide transcription profile analysis through Human U133 Puls 2.0 (Affymetrix) microarray

2015-01-23 | E-GEOD-65210 | biostudies-arrayexpress

Expression data from SMMC-7721 cells

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The effects of ETFA knockdown on the gene expression in SMMC-7721 human hepatoma cells

Project description:SMMC-7721 single clones stably expressing control shRNA or ETFA shRNAs 1# and 2# were generated and subjected to microarray.

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Long noncoding RNA expression profiles in sub-lethal heat treated hepatoma carcinoma cells. (SMMC-7721 cells: sub-lethal heat treated vs. untreated)

Project description:Transcriptional profiling of SMMC-7721 cells comparing control untreated with sub-lethal heat treated cells (50°C for 10 min). Differentially expressed lncRNA and mRNA were measured with an Agilent Human lncRNA+mRNA Array V4.0 (4 × 180 K format) containing 41,000 lncRNAs and 34,000 mRNAs. Goal was to determine the effects of sub-lethal heat treatment on global hepatoma carcinoma cells gene expression.

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Long non-coding RNAs and mRNAs regulated by TGF-M-NM-2

Project description:The role of TGF-M-NM-2-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination is well established, but the involvement of lncRNAs in TGF-M-NM-2 signaling is still unknown. In this study, we observed that the lncRNA-Activated by TGF-M-NM-2 (lncRNA-ATB) was upregulated in hepatocellular carcinoma (HCC) metastases and associated with poor prognosis. lncRNA-ATB promotes the invasion-metastasis cascade, which suggest that lncRNA-ATB, a mediator of TGF-M-NM-2 signaling, could predispose HCC patients to metastases and may serve as a potential target for anti-metastatic therapies. SMMC-7721 hepatoma cells were continuously treated with 10 ng/ml of recombinant TGF-M-NM-21 for 21 days. Total RNA recovered from three untreated cells and three treated cells were used to acquire different expression profiles of mRNAs and lncRNAs.

2014-02-08 | E-GEOD-54797 | biostudies-arrayexpress

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Project description:The whole human genome microarray was used for analysis of the differentially expressed genes in SMMC-7721 cells when exposed to 1 μM IMB5043 treatment for 24 h.

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