Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of Mammary Gland Tumor epithelial cells

ABSTRACT: Transcriptional profiling of mammary gland tumors from transgenic mice overexpressing the p110 or p75 isoform of CUX1. RNA was extracted from epithelial cells isolated using Laser-Capture Microdissection (LCM)

ORGANISM(S): Mus musculus

PROVIDER: GSE54804 | GEO | 2014/09/15

SECONDARY ACCESSION(S): PRJNA237722

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression profiling of Mammary Gland Tumor epithelial cells

Project description:Transcriptional profiling of mammary gland tumors from transgenic mice overexpressing the p110 or p75 isoform of CUX1. RNA was extracted from epithelial cells isolated using Laser-Capture Microdissection (LCM) 9 tumors from p75-CUX1 transgenic mice and 8 tumors from p110-CUX1 mice. Biological samples were hybridized in technical duplicates with dye swaps between duplicates against a common reference for normalization.

2014-09-15 | E-GEOD-54804 | biostudies-arrayexpress

Genomic studies controvert the existence of the CUX1 p75 isoform

Project description:CUX1, a homeodomain-containing transcription factor, is recurrently deleted or mutated in multiple tumor types. In myeloid neoplasms, CUX1 deletion or mutation carries a poor prognosis. We have previously established that CUX1 functions as a tumor suppressor in hematopoietic cells across multiple organisms. Others, however, have described oncogenic functions of CUX1 in solid tumors, often attributed to truncated CUX1 isoforms, p75 and p110. Given the clinical relevance, it is imperative to clarify these discrepant activities. Herein, we sought to determine the CUX1 isoforms expressed in hematopoietic cells, and find that they express the full-length p200 isoform. Through the course of this analysis, we found no evidence of the p75 alternative transcript in any cell type examined. Using an array of orthogonal approaches, including biochemistry, proteomics, CRISPR/Cas9 genomic editing, and analysis of functional genomics datasets across a spectrum of normal and malignant tissue types, we found no data to support the existence of the CUX1 p75 isoform generated by an alternative transcriptional start site. Based on these results, prior studies of p75 require reevaluation, including the interpretation of oncogenic roles attributed to CUX1.

2022-02-17 | PXD027527 | Pride

Sebaceous gland progenitor cell RNAseq during morphogenesis and steady state homeostasis

Project description:Laser capture microdissection enriched for sebaceous gland basal cells at P4, P10, P30

2019-07-15 | GSE126491 | GEO

Gene expression profiling of LCM captured breast cancer cells

2014-09-16 | GSE54002 | GEO

Laser Capture Microdissection-based RNAseq for Profiling Mouse and Human Sebaceous Gland Transcriptomes

Project description:We describe the use of laser capture microdissection (LCM) to isolate human and murine sebaceous glands (SGs) for transcriptomic analysis and publish this SG transcriptomic data for reference. We show that compared to whole skin RNA sequencing, LCM RNA sequencing allows for high resolution in identifying and describing SG genes at homeostasis. Lastly, we compare this LCM of sebaceous glands to published SG clusters from single cell RNA sequencing of skin, and show that we achieve greater resolution and depth with the LCM approach.

2024-01-26 | GSE230682 | GEO

Phellgoen (PH), aerenchymatous phellem (AP), and cortex (CO) specific microarray analysis in soybean

Project description:AP and phellogen gene expressions were compared with the cortex using microarrays. These tissues were isolated by laser capture microdissection (LCM).

2022-10-21 | GSE216072 | GEO

Gene expression profiling of LCM captured breast cancer cells

Project description:The purpose of this study is to obtain comprehensive gene expression profiles in breast cancer. Mammary gland cells were specifically isolated from 433 clinical tissue samples by laser capture microdissection (LCM). Total RNAs were extracted from LCM captured samples. We investigated gene expression profiles in 417 patients with breast cancer and 16 non-tumor tissues as a normal control using an Affymetrix GeneChip. Mammary gland cells were captured from clinical tissues of breast cancer patient by LCM. Gene expression profilings for 417 tumor and 16 non-tumor samples were acquired using GeneChip Human Genome U133 Plus 2.0 (Affymetrix, Santa Clara, CA). Microarray datasets were normalized and transformed to log2 values using robust multi-array average (RMA) method with R statistical software and BioConductor package.

2014-09-16 | E-GEOD-54002 | biostudies-arrayexpress

Mammary gland

Project description:Goat Mammary gland RNA-Seq (DGE)

| PRJNA342566 | ENA

Laser Capture Microdissection (LCM) analysis of mycorrhizal rice roots

Project description:Laser Capture Microdissection (LCM) analysis of mycorrhizal rice roots

| PRJNA492135 | ENA

Microarray of pancreatic cancer and healthy tissue

Project description:Gene expression analyses of pancreatic adenocarcinoma and adjacent ductal epithelia from the same patient using bulk vs LCM dissected samples. Our results indicate that laser capture microdissection (LCM) is necessary to identify differentially expressed genes that discriminate between PDAC and healthy pancreatic ductal tissue.

2014-12-31 | GSE27890 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data