Genomics

Dataset Information

41

Transcription profiling by array of wild type and arr1,10,12 mutant Arabidopsis seedlings treated with the cytokinin benzyladenine


ABSTRACT: Effect of the cytokinin BA on wt and arr1,10,12 mutant seedlings The type B Arabidopsis Response Regulators (ARRs) of Arabidopsis thaliana are transcription factors that act as positive regulators in the two-component cytokinin signaling pathway. We employed a mutant-based approach to perform a detailed characterization of the roles of ARR1, ARR10, and ARR12 in plant growth and development. The most pronounced phenotype was found in the arr1-3 arr10-5 arr12-1 triple loss-of-function mutant, which showed almost complete insensitivity to high levels of exogenously applied cytokinins. The triple mutant exhibited reduced stature due to decreased cell division in the shoot, enhanced seed size, increased sensitivity to light, altered chlorophyll and anthocyanin concentrations, and an aborted primary root with protoxylem but no metaxylem. Microarray analysis revealed that expression of the majority of cytokinin-regulated genes requires the function of ARR1, ARR10, and ARR12. Characterization of double mutants revealed differing contributions of the type B ARRs to mutant phenotypes. Our results support a model in which cytokinin regulates a wide array of downstream responses through the action of a multistep phosphorelay that culminates in transcriptional regulation by ARR1, ARR10, and ARR12. This data was originally made available through ArrayExpress under the accession number E-MEXP-1573. Overall design: A benzyladenine treatment of 10 uM or a control treatment with equal DMSO was applied to A thaliana seedlings which were either knock outs for ARR1,10,12 or wild type.

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: George E Schaller  

PROVIDER: GSE54870 | GEO | 2014-02-12

SECONDARY ACCESSION(S): PRJNA237909

REPOSITORIES: GEO

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Publications

Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development.

Argyros Rebecca D RD   Mathews Dennis E DE   Chiang Yi-Hsuan YH   Palmer Christine M CM   Thibault Derek M DM   Etheridge Naomi N   Argyros D Aaron DA   Mason Michael G MG   Kieber Joseph J JJ   Schaller G Eric GE  

The Plant cell 20080822 8


The type B Arabidopsis Response Regulators (ARRs) of Arabidopsis thaliana are transcription factors that act as positive regulators in the two-component cytokinin signaling pathway. We employed a mutant-based approach to perform a detailed characterization of the roles of ARR1, ARR10, and ARR12 in plant growth and development. The most pronounced phenotype was found in the arr1-3 arr10-5 arr12-1 triple loss-of-function mutant, which showed almost complete insensitivity to high levels of exogenou  ...[more]

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