Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA expression data from peripheral blood mononuclear cell in patients with type 1 diabetes compared with normal controls.

ABSTRACT: Type 1 diabetes mellitus (T1D) is a common autoimmune disease mediated by autoimmune attack against pancreatic b cells. It has been reported that dys-regulation of microRNAs (miRNAs) may contribute to the pathogenesis of autoimmune diseases, including T1D. This study sought to identify T1D associated miRNAs in the peripheral blood mononuclear cell (PBMC).

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE55099 | GEO | 2014/08/16

SECONDARY ACCESSION(S): PRJNA238537

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

microRNA expression data from peripheral blood mononuclear cell in patients with type 1 diabetes compared with normal controls.

Project description:Type 1 diabetes mellitus (T1D) is a common autoimmune disease mediated by autoimmune attack against pancreatic b cells. It has been reported that dys-regulation of microRNAs (miRNAs) may contribute to the pathogenesis of autoimmune diseases, including T1D. This study sought to identify T1D associated miRNAs in the peripheral blood mononuclear cell (PBMC). Peripheral blood mononuclear of newly diagnosed type1 diabetes patients and normal controls were purified by LymphoprepTm gradient purification according to the manufacturerM-bM-^@M-^Ys instructions (Axis-Shield PoC AS, Oslo, Norway) for futher microarray analysis.

2014-08-16 | E-GEOD-55099 | biostudies-arrayexpress

Expression data from peripheral blood mononuclear cell in patients with type 1 diabetes compared with normal controls

2014-08-16 | GSE55098 | GEO

Expression data from peripheral blood mononuclear cell in patients with type 1 diabetes compared with normal controls

Project description:Type 1 diabetes mellitus (T1D) is a common autoimmune disease mediated by autoimmune attack against pancreatic b cells.Dys-regualtion of the component of peripheral blood mononuclear cells (PBMCs), including T-cells and B-cells, and smaller amounts of NK cells and dendritic cells, have all been implicated in this process This study sought to identify T1D associated differently expressed genes in the peripheral blood mononuclear cell (PBMC). Peripheral blood mononuclear of newly diagnosed type1 diabetes patients and normal controls were purified by LymphoprepTm gradient purification according to the manufacturer’s instructions (Axis-Shield PoC AS, Oslo, Norway) for futher microarray analysis.

2014-08-16 | E-GEOD-55098 | biostudies-arrayexpress

Prevalence of inflammatory pathways over immuno-tolerance in peripheral blood mononuclear cells of recent-onset type 1 diabetes

Project description:Background Changes in innate and adaptive immunity occurring in/around pancreatic islets had been observed in peripheral blood mononuclear cells (PBMC) of Caucasian T1D patients by some, but not all researchers. The aim of our study was to investigate whether gene expression patterns of PBMC of the highly admixed Brazilian population could add knowledge about T1D pathogenic mechanisms. METHODS: We assessed global gene expression in PBMC from two groups matched for age, sex and BMI: 20 patients with recent-onset T1D (≤ 6 months from diagnosis, in a time when the autoimmune process is still highly active), testing positive for one or more islet autoantibodies and 20 islet autoantibody-negative healthy controls. RESULTS: We identified 474 differentially expressed genes between groups. The most expressed genes in T1D group favored host defense, inflammatory and anti-bacterial/antiviral effects (LFT, DEFA4, DEFA1, CTSG, KCNMA1) and cell cycle progression. Several of the downregulated genes in T1D target cellular repair, control of inflammation and immune tolerance. They were related to T helper 2 pathway, induction of FOXP3 expression (AREG) and immune tolerance (SMAD6). SMAD6 expression correlated negatively with islet ZnT8 antibody. The expression of PDE12, that offers resistance to viral pathogens was decreased and negatively related to ZnT8A and GADA levels. The increased expression of long non coding RNAs MALAT1 and NEAT1, related to inflammatory mediators, autoimmune diseases and innate immune response against viral infections reinforced these data CONCLUSIONS: Our analysis suggested the activation of cell development, anti-infectious and inflammatory pathways, indicating immune activation, whereas immune-regulatory pathways were downregulated in PBMC from recent-onset non-Caucasian T1D patients with a different genetic profile.

2022-01-08 | GSE193273 | GEO

Transcriptional profile of peripheral blood mononuclear cells from Gestational Diabetes Mellitus Patients

Project description:The oligo microarray were used to determine gene expression profile of PBMC from gestational diabetes mellitus (GDM) patients.

2012-08-25 | E-MEXP-3349 | biostudies-arrayexpress

Assessing the role of microRNAs in post-transcriptional control of thymocytes and peripheral lymphocytes in NOD mice

Project description:As early as one month of age, non-obese diabetic (NOD) mice feature pancreatic infiltration of autoreactive T lymphocytes, which destruct insulin-producing beta cells, leading to autoimmune diabetes mellitus (T1D) within eight months. Thus, we hypothesized that during the development of T1D, the transcriptional modulation of immune reactivity genes, as well as, modulation of microRNAs (miRNAs) may occur during thymocytes mature into peripheral CD3+ T lymphocytes. Our aim is to analyze the transcriptional modulation of mRNA and microRNAs during development of thymocytes into peripheral CD3+ T lymphocytes in the context of the emergence of T1D.

2011-07-01 | E-MEXP-3235 | biostudies-arrayexpress

Inflammatory pathways in peripheral blood expression profile of recent-onset type 1 diabetes

Project description:Changes in innate and adaptive immunity occurring in and around pancreatic islets can also be observed in peripheral blood mononuclear cells (PBMC) of T1D patients in Caucasians. The aim of our study was to investigate whether gene expression patterns of PBMC could complement islet autoantibodies for T1D pathogenic mechanisms in the higlty admixed Brazilian population. Methods: We assessed global gene expression in PBMC from two groups mached for age, sex and BMI: The T1D group with 20 patients with recent-onset T1D (≤ 6 months from diagnosis, in a time when the autoimmune process is still highly active), testing positive for one or more islet autoantibodies and 20 islet autoantibody-negative healthy controls (Control group). Results: we identified 474 differentially expressed genes between groups. The most expressed genes in T1D group were mainly related to host defense, with inflammatory and anti-bacterial/antiviral effects ( LFT, DEFA4, DEFA1, CTSG, KCNMA1) as well as to cell cycle progression. Several of the downregulated genes in T1D influenced cellular repair, control of inflammation and immune tolerance. They were related to T helper 2 pathway, induction of FOXP3 expression (AREG) and immune tolerance (SMAD6). Conclusion: Our analysis suggested the activation of cell cycle/proliferation, anti-infectious and inflammatory pathways, indicating immune activation, whereas immunoregulatory pathways were downregulated in PBMC from recent-onset T1D patients. These alterations were elicited by a new genetic profile

2022-01-01 | GSE156035 | GEO

Transcriptional profile of peripheral blood mononuclear cells PBMC from type 1 Diabetes Mellitus Patients

Project description:The oligo microarray were used to determine gene expression profiles of PBMC from type 1 diabetes mellitus (T1DM) patients.

2012-08-24 | E-MEXP-3348 | biostudies-arrayexpress

Transcriptional Profiles of peripheral blood mononuclear cells - PBMC - from Type 2 diabetes mellitus Patients

Project description:The oligo microarrays were used to determine gene expression profiles of PBMC from type 2 diabetes mellitus (T2DM) patients.

2012-06-30 | E-MEXP-3287 | biostudies-arrayexpress

Transcriptional Profiles of peripheral blood mononuclear cells - PBMC - from Type 2 diabetes mellitus Patients

Project description:The oligo microarrays were used to determine gene expression profiles of PBMC from type 2 diabetes mellitus (T2DM) patients.

2015-04-01 | E-MTAB-2854 | biostudies-arrayexpress