Project description:AML3 cells were treated with Azacytidine and compared against untreated cells We used RNA-Seq to detail the global programme of gene expression in human untreated and Azacytidine treated AML3 cells
Project description:AML3 cells were treated with Azacytidine and compared against untreated cells Examination of methylation status in human untreated and Azacytidine treated AML3 cells
Project description:DNA microarray experiments were used to compare gene expression profiles of untreated and 5-azacytidine treated Escherichia coli at both logarithmic phase and early stationary phase The goal was to determine the effect of cytosine DNA methylation loss on gene expression (5-azacytidine is a methylation inhibitor)
Project description:Acute myeloid leukemia (AML) remains a challenging hematologic malignancy, necessitating the exploration of novel treatment strategies. In this study, we investigated the effects of all-trans retinoic acid (ATRA), AM80, and their combination treatments with GSK-LSD1 and MB3 on gene expression in OCI-AML3 cells.
Project description:Chromatin accessibility at LTR12C family has been shown to b required to maintain stemness properties in leukemic stem cells. Here using CRISPRi epigenetic silencing strategy, we targetted LTR12C family in OCI-AML3 cell line. Our ATAC-seq and CUT&RUN data demonstrate efficient targeting and silencing of entire family using only 4 sgRNAs. Epigenetic silencing of this family leads to slower growth in targeted OCI-AML3 cells compared to control cells.