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Transcriptome profiling of alkaloid biosynthesis induced opium poppy

ABSTRACT: To identify the molecular basis of genome-wide transcriptome analysis in induced opium poppy capsule tissues NimbleGen microarray (12X135 K element) analysis was performed. Fungal elicitor methyl jasmonate (MeJa) treatment was applied on capsules of opium poppy during 0, 3, and 12 h.

ORGANISM(S): Papaver somniferum

PROVIDER: GSE55196 | GEO | 2014/02/21

SECONDARY ACCESSION(S): PRJNA238882

REPOSITORIES: GEO

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Transcriptome profiling of alkaloid biosynthesis induced opium poppy

Project description:To identify the molecular basis of genome-wide transcriptome analysis in induced opium poppy capsule tissues NimbleGen microarray (12X135 K element) analysis was performed. Fungal elicitor methyl jasmonate (MeJa) treatment was applied on capsules of opium poppy during 0, 3, and 12 h. Four sample sets were prepared for array analyses: i) control ii) 0 hour after treatment iii) 3 hour after treatment iv) 12 h after treatment

2014-02-21 | E-GEOD-55196 | biostudies-arrayexpress

Regulation of alkaloid biosynthesis by miRNAs in opium poppy

Project description:MicroRNAs (miRNA) are ~21 nucleotide long, small endogenous non-coding RNAs that functioning in regulation of gene expression found in many eukaryotes. In this study, small RNA libraries of opium poppy from four different tissues (leaf, root, capsule, stem) were sequenced using high-throughput next generation Illumina sequencing (Solexa) technology to investigate potential mode of actions of miRNAs in alkaloid biosynthesis. A total of 27 opium poppy miRNAs which have roles in regulation of alkaloid biosynthesis were identified in this study. A six chip study using miRNA isolated from four separate tissues (capsule, leaf, stem, root). small RNA libraries of opium poppy tissues were sequenced using high-throughput next generation Illumina sequencing (Solexa) technology to investigate potential mode of actions of miRNAs in alkaloid biosynthesis. Furthermore, the novel opium poppy miRNAs were also confirmed by a direct small RNA cloning strategy. The microarray platform were performed to measure and analyze the mirnome of the different opium poppy tissues.

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Regulation of alkaloid biosynthesis by miRNAs in opium poppy

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Project description:To facilitate genome annotation, we performed RNA sequencing of seven different opium poppy tissues (leaf, petal, stamen, capsule, stem, fine root, tap root).

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Transcriptome analysis of Opium poppy using Illumina HiSeq X Ten platform [cultivars]

Project description:Purpose: Opium poppy is one of the most important medical plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about its regulation mechanism in benzylisoquinoline alkaloids (BIAs) biosynthesis. Herein, the Transcriptome dataset of Opium poppy was constructed to identify the gene involved in its regulation mechanism in BIAs biosynthesis. Methods: Using Illumina HiSeq X Ten platform, 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars were constructed. Results: The high-quality transcripts were subsequent quantified with the short reads, and the expression of each unigenes among different samples was calculated by RPKM (the reads per kilobase per million mapped reads). Conclusions: These data provide a foundation of opium poppy transcriptome which may contribute to understand the regulation of BIAs biosynthesis.

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Transcriptome analysis of Opium poppy using Illumina HiSeq X Ten platform [different tissues]

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Proteomic analysis of drought stress response in Opium poppy

Project description:Main aim of the project is to analyze differentially expressed genes and proteins in early germination stages of Opium poppy plants under drought stress conditions. These results will be used for selection of drought resistant varieties for dry regions and also for further breeding of drought resistant varieties. Side aim of the project is to utilize standard set of methods for sample preparation and processing for proteomic and transcriptomic analysis, that would lead to more comparable results for future assessments in the field of poppy drought stress resistance research.

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Project description:Tissue-specific transcriptomic profiling of opium poppy (Papaver somniferum)

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