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Expression data from 1,25(OH)2D treated human testicular cells

ABSTRACT: Human testicular cells were isolated mechanically and enzymatically from testis of braindead donors and from urological samples. The expression of genes was studied at baseline and 1,25(OH)2D treated conditions. We used microarrays to analyze the gene expression underlying vitamin D metabolism in human testis cells and identified distinct classes of up-regulated genes during this process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE55207 | GEO | 2014/03/14

SECONDARY ACCESSION(S): PRJNA238894

REPOSITORIES: GEO

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Expression data from 1,25(OH)2D treated human testicular cells

Project description:Human testicular cells were isolated mechanically and enzymatically from testis of braindead donors and from urological samples. The expression of genes was studied at baseline and 1,25(OH)2D treated conditions. We used microarrays to analyze the gene expression underlying vitamin D metabolism in human testis cells and identified distinct classes of up-regulated genes during this process. Testicular primary cells were treated with 100nM 1,25(OH)2D for 24h and gene expression studied by microarray on transcript level.

2014-03-14 | E-GEOD-55207 | biostudies-arrayexpress

Time course of 1,25(OH)2D treated RWPE1 cells.

Project description:Background: Prostate cancer is the second leading cause of cancer mortality among US men. Epidemiological evidence suggests that high vitamin D status protects men from prostate cancer and the active form of vitamin D, 1α,25 dihydroxyvitamin D3 (1,25(OH)2D) has anti-cancer effects in cultured prostate cells. Still, the molecular mechanisms and the gene targets for vitamin D-mediated prostate cancer prevention are unknown. Results: We examined the effect of 1,25(OH)2D (+/- 100 nM, 6, 24, 48 h) on the transcript profile of proliferating RWPE1 cells, an immortalized, non-tumorigenic prostate epithelial cell line that is growth arrested by 1,25(OH)2D (Affymetrix U133 Plus 2.0, n=4/treatment per time and dose). Our analysis revealed many transcript level changes at a 5% false detection rate: 6 h, 1571 (61% up), 24 h, 1816 (60 % up), 48 h, 3566 (38 % up). 288 transcripts were regulated similarly at all time points (182 up, 80 down) and many of the promoters for these transcripts contained putative vitamin D response elements. Functional analysis by pathway or Gene Set Analysis revealed early suppression of WNT, Notch, NF-kB, and IGF1 signaling. Transcripts related to inflammation were suppressed at 6 h (e.g. IL-1 pathway) and suppression of proinflammatory pathways continued at later time points (e.g. IL-17 and IL-6 pathways). There was also evidence for induction of anti-angiogenic pathways and induction of transcripts for protection from oxidative stress or maintenance of cell redox homeostasis at 6 h. Conclusions: Our data reveal of large number of potential new, direct vitamin D target genes relevant to prostate cancer prevention. In addition, our data suggests that rather than having a single strong regulatory effect, vitamin D orchestrates a pattern of changes within prostate epithelial cells that limit or slow carcinogenesis.

2009-12-18 | GSE15947 | GEO

1,25(OH)2D3 upregulated lncRNA

Project description:1,25(OH)2D3 upregulated lncRNA

| PRJNA741924 | ENA

1,25(OH)2D3 up-regulated lncRNA

Project description:1,25(OH)2D3 up-regulated lncRNA

| PRJNA740034 | ENA

RNA-seq 1,25(OH)2D3 time course in THP-1 cells

Project description:gene expression profiling by RNA-seq in THP-1 cells treated with 1,25(OH)2D3 for 2.5-24 h three independent experiments of 1,25(OH)2D3 time course in THP-1 cells

2016-07-04 | E-GEOD-69284 | biostudies-arrayexpress

FAIRE-seq 1,25(OH)2D3 time course in THP-1 cells

Project description:Assessment of regions of open chromatin by FAIRE-seq in THP-1 cells treated with 1,25(OH)2D3 for 0-48 h Three independent experiments of 1,25(OH)2D3 time course in THP-1 cells

2016-07-04 | E-GEOD-69297 | biostudies-arrayexpress

Investigation of the effect of 1,25(OH)2D3 on murine CD11+ dendritic cells

Project description:Gene expression was compared between CD11c+ bone marrow derived dendritic cells (BMDC) was compared between cells conditioned in 20nM 1,25(OH)2D3 (Vitamin D) or vehicle. A second analysis compared gene expression in 1,25(OH)2D3 or vehicle CD11c+ BMDCs which were matured in presence of 0.1ug/ml LPS.

2019-03-13 | GSE114768 | GEO

RNA-seq 1,25(OH)2D3 time course in THP-1 cells

Project description:gene expression profiling by RNA-seq in THP-1 cells treated with 1,25(OH)2D3 for 2.5-24 h

2016-01-02 | GSE69284 | GEO

RNA expression in primary huamn muscle cells treated with 1,25(OH)2D3 or vehicle

Project description:We report the effects of 1,25(OH)2D3 treatment on the mRNA expression in human muscle cells Primary cultures of human muscle cells were treated with 1,25(OH)2D3 or vehicle for 48 hours.

2015-11-24 | E-GEOD-68323 | biostudies-arrayexpress

microRNA expression in primary human muscle cells treated with 1,25(OH)2D3 or vehicle

Project description:We report the effects of 1,25(OH)2D3 treatment on the microRNA expression in human muscle cells Primary cultures of human muscle cells were treated with 1,25(OH)2D3 or vehicle for 48 hours.

2015-11-24 | E-GEOD-70933 | biostudies-arrayexpress

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