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Small RNA profile in Moso Bamboo root and leaf

ABSTRACT: The small RNA libraries from Moso bamboo (Phyllostachy heterocycla) roots and leaves were constructed by using high definition adapters . The small RNA profiles were analyzed. A collection of micro RNAs with similarity to the micro RNA entries in mirbase were discovered. The putative genomic loci of the micro RNAs were identified.

ORGANISM(S): Phyllostachys edulis

PROVIDER: GSE55305 | GEO | 2014/08/01

SECONDARY ACCESSION(S): PRJNA239249

REPOSITORIES: GEO

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Small RNA profile in Moso Bamboo root and leaf

Project description:The small RNA libraries from Moso bamboo (Phyllostachy heterocycla) roots and leaves were constructed by using high definition adapters . The small RNA profiles were analyzed. A collection of micro RNAs with similarity to the micro RNA entries in mirbase were discovered. The putative genomic loci of the micro RNAs were identified. Analysis of small RNA profiles from the root and leaf tissues of young Moso Bamboo seedlings

2014-08-01 | E-GEOD-55305 | biostudies-arrayexpress

Genome-wide profiling of circular RNAs in rapidly growing culms of moso bamboo

Project description:Transcriptome analysis of circular RNAs in the fast-growing species moso bamboo

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Single-cell transcriptome atlas of Moso bamboo

Project description:Bamboo is a large Poaceae perennial with 1,642 species worldwide. We reported single-cell transcriptomes of 14,279 filtered single cells derived from the basal root tips of Moso bamboo.

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Distinct transcriptome of moso bamboo in the phase transition from vegetative to reproductive growth

Project description:Bamboo represents the only major lineage of grasses that is native to forests and is one of the most important non-timber forest products in the world. Moso bamboo is a large woody bamboo that has ecological, economic and cultural value in Asia and accounts for ~70% of the total bamboo growth area (Peng et al., 2013). In the aspect of epigenetics of Moso bamboo,the total genomic DNA methylation rates in Moso bamboo at different chronological ages were significantly different (Yuan et al., 2014). Those show that the flowering of Moso bamboo are closely related to epigenetic modification. However, DNA methylation in single base resolution has never been reported in moso bamboo. In this study, leaves from three-week bamboo, one-year bamboo, flower in next year bamboo, flowering bamboo and Flower florets was used for bisulfite sequencing (BS-seq), and RNA-Seq. Genome-wide methylation profile and gene expression analysis were constructed to reveal the factors to regualte the phase transition from vegetative to reproductive growth in moso bamboo.

2021-04-30 | GSE121216 | GEO

Distinct DNA methylomes of moso bamboo in the phase transition from vegetative to reproductive growth

2021-04-30 | GSE122135 | GEO

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Project description:moso bamboo Raw sequence reads

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Data-independent acquisition proteomics reveals the effects of red and blue light on the growth and development of moso bamboo(Phyllostachys edulis) seedlings

Project description:Moso bamboo is a fast-growing bamboo species with high economic, social and cultural value. The method of transplanting moso bamboo seedlings for afforestation has become a more economical and effective method. The effect of light on the growth of plant seedlings is mainly reflected in the regulation of different light quality on the growth and development of seedlings, including light morphogenesis, photosynthesis and secondary metabolites. Therefore, studying the effects of specific wavelength light on the physiology and proteome of moso bamboo seedlings will play an important role in growing seedlings and seed cultivation of moso bamboo. Here, moso bamboo seeds were germinated in the dark and then were transferred to the blue and red-light conditions. After 14 days, we observed the effects of different light treatments on the growth and development of seedlings, and then compared and analyzed their proteome.

2023-05-10 | PXD039570 | Pride

Genome-wide identification and transcriptomic profiling of auxin concentration and signaling related genes in moso bamboo (Phyllostachys heterocycla)

Project description:Auxin is essential for plant growth and development by altering downstream gene expression. Although large progresses have been made on auxin-concentration, distribution and signaling pathways in model plants like Arabidopsis and rice, little is known in moso bamboo which belongs to grass family, and has great economic and social value. Here we performed genome-wide analysis of the key components related to auxin action, and identified 13 YUCCA genes for auxin synthesis, 14 PIN-FORMED/PIN-like (PIN/PILS) proteins 7 AUXIN1/LIKE-AUX1 (AUX1/LAX) family members for auxin transport, 10 auxin binding factors (AFB) for auxin perception, 43 auxin/indole-3-aceticacid (AUX/IAA) and 41 auxin response transcription factors (ARF) genes for auxin signaling in moso bamboo genome. We further performed phylogenetic analysis of those auxin action related genes from Arabidopsis, Oryza sativa and moso bamboo. To know those genes’ ability to response exogenous auxin and to generate a comprehensive transcriptome overview of auxin response in moso Bamboo, we performed RNA_seq analysis. Our data showed that auxin regulates genes related its biosynthesis, transport, signaling. Moreover, we present the interaction between auxin and other phytohormones at the level of transcription. In summary, we identified the key gene families involved in the auxin action pathways in moso bamboo, and generated a transcriptional overview of the auxin response in moso bamboo. Our data open up an opportunity to uncover the precise roles of auxin action pathways in this important species.

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Transcriptome analysis of Gibberellin-response gene in the fast-growing species moso Bamboo

Project description:Moso bamboo is well known with its rapid growth rate. In order to shed light on the underlying mechanism of growth, we applied Gibberellin (GA) to mimic the fast development process in bamboo and found that exogenous GA can significantly increase the length of internode of moso bamboo. Subsequently, we sequenced the transcriptome by high-through sequencing and identified 5,148 different expressed genes (DEG) and find out 22 genes that take part in GA signaling pathway is significantly differently expressed, including GID1A, GID1B and GAI. From GO analysis, a group of genes related to response hormone stimulus are enriched in up-regulated DEGs and 178 GA-responded genes are involved in other plant hormone signaling pathway, of which genes MYB74 and NCED5 are belong to both two pathways. Besides, clusters of genes about photosynthesis are enriched in down-regulated DEGs. By looking into the content of chlorophyll, we find out that the concentrations of chlorophyll are obviously decreased which verify that photosynthesis do weakened after GA treatment. Through method of slicing by hand, it appears that an increased condensation of lignin after GA treatment. The results imply a functional role of GA in bamboo growth, and maybe major in cell wall formation, and photosynthesis. Differential AS alternative splicing (AS) events upons GA induce suggested that post-transcriptional regulation is involved in the GA response.

2018-06-27 | GSE104596 | GEO

Transcriptomic profiling of brassinosteroids response genes in moso bamboo (Phyllostachys heterocycla)

Project description:Brassinosteroids (BRs) are a group of plant steroid hormones that play crucial roles various of growth and developmental processes. Biological function and signal transduction pathway of BR has been well characterized in model plants like Arabidopsis and rice. However, their biological roles are still unclear in tree species, especially in an important non-timber plant moso bamboo, which has great ecological and economic value and distinguish fast-growth feature. Here we reported that reduce endogenous brassinosteroid by biosynthesis inhibitor propiconazole reduced both root and shoot growth in seedling stage. Exogenous BR application promoted shoot bract elongation and inclination of lamina joint and bract. Genome-wide transcriptome analysis were performed to identify hundreds of differential expressed genes by BR and propiconazole treatment in shoot and root parts of bamboo seedling, respectively. GO analysis revealed that BR regulates cell wall related genes, hydrogen peroxide catabolic genes and auxin related genes to promote bamboo root development and elongation. Our study identified BR response genes and provides a comprehensive resource for molecular mechanism research of bamboo growth.

2018-12-11 | GSE123529 | GEO

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