Systematic identification of regulatory elements in conserved 3’-untranslated regions of human transcripts

ABSTRACT: We present a combined experimental/computational technology to reveal a catalogue of functional regulatory elements embedded in 3’UTRs of human transcripts. We used a bidirectional reporter system coupled with flow cytometry and high-throughput sequencing to measure the effect of short, non-coding vertebrate-conserved RNA sequences on transcript stability and translation. Information-theoretic motif analysis of the resulting sequence-to-gene-expression mapping revealed linear and structural RNA cis-regulatory elements that positively and negatively modulate the post-transcriptional fates of human transcripts.

ORGANISM(S): Homo sapiens

PROVIDER: GSE55396 | GEO | 2014/03/21

SECONDARY ACCESSION(S): PRJNA239465

REPOSITORIES: GEO