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Genes affected by SFRP5 overexpression in LSK cells and CLP

ABSTRACT: To understand gene expression signatures between wild-type and SFRP5-overexpressing cells

ORGANISM(S): Mus musculus

PROVIDER: GSE55646 | GEO | 2014/03/20

SECONDARY ACCESSION(S): PRJNA240285

REPOSITORIES: GEO

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Genes affected by SFRP5 overexpression in LSK cells and CLP

Project description:To understand gene expression signatures between wild-type and SFRP5-overexpressing cells To examine genes regulated by SFRP5 expression, sets of microarrays were conducted with Lin- c-kitHi Sca-1+ cells and Lin- c-kitLo Sca-1Lo IL7RÎ±+ CLP-enriched cells derived from adult bone marrow of SFRP5-transgenic mice or their WT littermates.

2014-03-20 | E-GEOD-55646 | biostudies-arrayexpress

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DEK1 calpain overexpression

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Genes affected by PAD4 deficiency in LSK cells obtained from mouse bone marrow.

Project description:We found PAD4, which is one of the transcriptional co-regulator by histone modification, was highly expressed in lineage-, Sca-1+, c-kit+ (termed as LSK) cells of mouse bone marrow. To find the target genes which are regulated by PAD4 in LSK cells, we analyzed gene expression in PAD4-deficient mouse as compared with wild-type mouse.

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DEK1 calpain overexpression

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2011-03-08 | E-GEOD-27701 | biostudies-arrayexpress

Genes affected by PAD4 deficiency in LSK cells obtained from mouse bone marrow.

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Overexpression of subtilate4.13

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Effect of ablation of Sfrp5 on gene expression in gonadal white adipose tissue

Project description:We identified secreted frizzled-related protein-5 (Sfrp5) as a transcript that is upregulated during adipocyte differentiation and that is increased in white adipose tissue (WAT) of obese mice, compared to lean mice. To investigate the function of sFRP5 in adipose tissue biology, we studied sFRP5Q27stop mice, in which ENU mutagenesis was used to create a premature stop codon at Gln27, thereby creating a likely null allele. Male wild-type or Sfrp5 KO (Q27Stop) mice were fed a high-fat diet from the ages of four weeks to twelve weeks. At twelve weeks of age, mice were euthanized. Total RNA was then isolated from gonadal WAT and RNA was analyzed by Affymetrix microarrays. Seven wild-type and eight Sfrp5 KO mice were used.

2012-04-24 | E-GEOD-37514 | biostudies-arrayexpress

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