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Influence of SB431542 on J1 mouse embryonic stem cells growth

ABSTRACT: To know exactly how SB431542 contribute to mouse embryonic stem cells (mESCs) undifferentiated state maintenance, microarray experiment for DMSO mock treated and SB431542 treated J1 mESCs was performed

ORGANISM(S): Mus musculus

PROVIDER: GSE55729 | GEO | 2014/10/29

SECONDARY ACCESSION(S): PRJNA240819

REPOSITORIES: GEO

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Influence of SB431542 on J1 mouse embryonic stem cells growth

Project description:To know exactly how SB431542 contribute to mouse embryonic stem cells (mESCs) undifferentiated state maintenance, microarray experiment for DMSO mock treated and SB431542 treated J1 mESCs was performed J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented without or with SB431542 for 24 hours, then total RNA was extracted for analysis.

2014-10-29 | E-GEOD-55729 | biostudies-arrayexpress

mRNA profiling of mouse embryonic cells with activin or SB431542 treatment

Project description:We analysed the mRNA expression profiles of control and VprBP knockdown mouse embryonic stem cells (mESCs) with or without Activin treatment or TGF-β/Activin inhibitor SB431542 challenge for 4 days during differentiation

2016-09-10 | GSE86631 | GEO

Deep sequencing identifies PD0325901 modulated microRNAs in the J1 mESCs

Project description:PD0325901 is involved in improving reprogramming efficiency during inducing pluripotent stem cells (iPSC) or maintain a blastocyst-like state in embryonic stem cells (ESC). However, the knowledge about this small molecule regulating miRNAs in ESC was limited. To understand the role of miRNAs during PD03-induced ESC maintenance and gain an insight how PD0325901 regulates miRNAs expression; we performed small RNA sequencing using Illumina HiSeq 2000 under the compounds treatment. The data show the miRNAs regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented with PD0325901 for 24 hours, J1 treated with DMSO was set as control. Then total RNA was extracted for analysis.

2016-05-17 | E-GEOD-67570 | biostudies-arrayexpress

Comparison of Gene Expression Profiles of J1 ES and J1 EB Cells.

Project description:Comparison of gene expression profiles of J1 embryonic stem cells and J1 embryoid bodies. This study should reveal genes that, when expressed, are responsible for the maintenance of the stem cell phenotype. Keywords: other

2005-09-01 | GSE3223 | GEO

Identifying PD0325901-regulated genes in the J1 mESCs

Project description:It has been demonstrated that PD0325901 promotes self-renewal of mouse embryonic stem cells, however, the target genes of PD0325901 are not fully understood. To better understand the downstream target genes of PD0325901, we performed Microarray analyses to identify their downstream targets. The data show the genes regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented without or with PD0325901 for 24 hours, then total RNA was extracted for analysis.

2016-05-17 | E-GEOD-67534 | biostudies-arrayexpress

AICAR can modulate the expression of microRNA in J1 mESC

Project description:We have demonstrated AICAR can maintain J1 mouse ES cells pluripotency in our previous research, yet its effects on ES cells miRNAs expression remain a mystery. In this study, we performed small RNA (sRNA) High-throughput sequencing using Illumina HiSeq 2000 to investigate the influence of AICAR on J1 mouse ES cells miRNAs expression and further found the mechanism of how miRNAs affect ES cells pluripotency maintenance. Samples that treated by DMSO is used as a control.

2014-02-06 | GSE54586 | GEO

Metallosphaera sp. J1 strain:J1

Project description:Metallosphaera sp. J1 strain:J1 Genome sequencing and assembly

| PRJNA799479 | ENA

Cellulosimicrobium cellulans J1 strain:J1

Project description:Cellulosimicrobium cellulans J1 strain:J1 Genome sequencing and assembly

| PRJNA205968 | ENA

Transcription profiling of mouse J1 ES and J1 EB cells

2008-11-06 | E-GEOD-3223 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of primary cultured astrocytes treated with SB431542, Ro5-3335 and HLM006474 inhibitors.

Project description:Purpose: The goals of this study are to investigate differential genes expression with RNA-seq in primary cultured astrocytes treated with the Tgf-beta1 receptor inhibitor SB431542, the Runx1 inhibitor Ro5-3335 and the E2f1 inhibitor HLM006474. Methods: Primary cultured astrocytes were isolated from either C57BL/6 wild-type neonatal mice. Total RNA was extracted from SB431542, Ro5-3335 and HLM006474-treated astrocytes with the RNeasy Plus Micro Kit (Qiagen; 74034). mRNA profiles were generated by deep sequencing using Illumina NovaSeq 6000. The sequence reads that passed quality filters were aligned to the mouse reference genome (GRCm38) using the STAR software package. Differential expression analysis were performed using the R package DESeq2. qPCR validation was performed using SYBR Green assays. Results: After quality control and data filtering, about 50 million raw reads per sample were obtained. We mapped sequence reads per sample to the mouse reference genome (GRCm38) and identified 35825 transcripts vehicle, SB431542, Ro5-3335 and HLM006474 group. Differentially expressed genes (DEGs) were analyzed with a fold change ≥1.5 and p value <0.05. A total of 748 DEGs were identified in SB431542 group with 347 upregulated genes and 401 downregulated genes. A total of 397 DEGs were identified in Ro5-3335 group with 82 upregulated genes and 315 downregulated genes. A total of 856 DEGs were identified in HLM006474 group with 216 upregulated genes and 640 downregulated genes. Hierarchical clustering heat map, pathway enrichment and analysis of DEGs were performed. Conclusions: Our RNA-seq dataset represents the differentially expressed genes in the C57BL/6 astrocytes treated with SB431542, Ro5-3335 and HLM006474-treated astrocytes.

2022-11-23 | GSE174523 | GEO

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