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Identification of transcripts whose abundance changes in response to acute switches in DSX isoform

ABSTRACT: RNAseq of dissected adult fat body after DSX isoform is switched using temperature inducible alleles or constructs.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE55848 | GEO | 2014/10/30

SECONDARY ACCESSION(S): PRJNA241132

REPOSITORIES: GEO

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Identification of transcripts whose abundance changes in response to acute switches in DSX isoform

Project description:RNAseq of dissected adult fat body after DSX isoform is switched using temperature inducible alleles or constructs. Biological duplicates were sequenced for each genotype at each condition.

2014-10-30 | E-GEOD-55848 | biostudies-arrayexpress

Identification of transcripts whose abundance changes in response to acute switches in DSX isoform in the ovary and testis

Project description:RNAseq of dissected adult ovary and testis after DSX isoform is switched using temperature inducible alleles or constructs.

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Identification of transcripts whose abundance changes in response to acute switches in DSX isoform in the ovary and testis

Project description:RNAseq of dissected adult ovary and testis after DSX isoform is switched using temperature inducible alleles or constructs. Biological duplicates were sequenced for each genotype at each condition.

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Nucleoporin107 mediates female sexual differentiation via Dsx

Project description:We recently identified a missense mutation in Nucleoporin107 (Nup107; D447N) underlying XX-ovarian-dysgenesis (XX-OD), a disorder characterized by underdeveloped and dysfunctional ovaries. Specific knockdown of Nup107 in the somatic gonadal cells and moreover, modelling of the human mutation in Drosophila result in ovarian-dysgenesis-like phenotypes in female flies. The aberrant phenotypes in larval and adult ovaries compromised for Nup107 are associated with hyperactivation of BMP signalling. Transcriptomic analysis identified the somatic sex-determination gene Doublesex (dsx) and the extracellular metalloprotease AdamTS-A as targets of Nup107. Either loss or gain of Dsx in the gonadal soma is sufficient to respectively mimic or rescue the phenotypes induced by Nup107 loss. Furthermore, adamTS-A is transcriptionally regulated by Dsx, and its knockdown in the somatic gonad hyperactivates BMP signaling and to a large extent recapitulates loss of Nup107 phenotypes. Thus, Dsx acts downstream of Nup107 to impact female germline stem cells via sex-specific modulation of the BMP pathway.

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Identifying Targets of DSX with ChIP-seq

Project description:We have used ChIP-seq on Drosophila S2 cells to characterize genome-wide DSX occupancy.

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Identifying targets of DSX with DamID-chip

Project description:We have used microarrays to identify DSX occupancy signal in adult female fatbody using the DamID protocol.

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Identifying targets of DSX with DamID-chip

Project description:We have used microarrays to identify DSX occupancy signal in adult female fatbody using the DamID protocol. We have performed DamID-chip on adult female fatbody with three biological replicates.

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Project description: Not available

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Identifying targets of DSX with DamID-seq

Project description:We have adapted the DamID protocol for use with high throughput sequencing. We have used DamID to identify the positions within the Drosophila genome where the transcription factor DSX is bound. We sequenced DpnI-digested genomic DNA from fly tissues containing UAS-Dam (control) or UAS-Dam-DsxF or UAS-Dam-DsxM.

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