Transcriptomics,Genomics

Dataset Information

24

Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans


ABSTRACT: The aim of the study was to investigate how short-term fasting affects whole-body energy homeostasis and skeletal muscle energy/nutrient-sensing pathways and transcriptome in humans. For this purpose, twelve young healthy men were studied during a 24-hour fast. Skeletal muscle biopsies were collected and analyzed at baseline and after 4, 10 and 24h of fasting. As expected, fasting induced a time-dependent decrease in plasma insulin and leptin levels, whereas levels of ketone bodies and free fatty acids increased. This was associated with a metabolic shift from glucose towards lipid oxidation. Transcriptome profiling identified genes that were significantly regulated by fasting in skeletal muscle at both early and late time-points. Collectively, our study provides a comprehensive map of the main energy/nutrient-sensing pathways and transcriptomic changes during short-term adaptation to fasting in human skeletal muscle Overall design: Total RNA obtained from skeletal muscle biopsies collected at 1.5, 4 10 and 24h post-meal in healthy men subjected to a 24h-fast

INSTRUMENT(S): Illumina HumanHT-12 V4.0 expression beadchip

SUBMITTER: Bruno Guigas  

PROVIDER: GSE55924 | GEO | 2014-12-02

SECONDARY ACCESSION(S): PRJNA241325

REPOSITORIES: GEO

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Publications

Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans.

Wijngaarden Marjolein A MA   Bakker Leontine E H LE   van der Zon Gerard C GC   't Hoen Peter A C PA   van Dijk Ko Willems KW   Jazet Ingrid M IM   Pijl Hanno H   Guigas Bruno B  

American journal of physiology. Endocrinology and metabolism 20140923 10


During fasting, rapid metabolic adaptations are required to maintain energy homeostasis. This occurs by a coordinated regulation of energy/nutrient-sensing pathways leading to transcriptional activation and repression of specific sets of genes. The aim of the study was to investigate how short-term fasting affects whole body energy homeostasis and skeletal muscle energy/nutrient-sensing pathways and transcriptome in humans. For this purpose, 12 young healthy men were studied during a 24-h fast.  ...[more]

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