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Streptococcus mutans UA159 grown in the presence of glucose or maltose pH7 and pH5

ABSTRACT: Transcriptional analysis of the effects of sugar source in S. mutans

ORGANISM(S): Streptococcus mutans UA159 Streptococcus mutans

PROVIDER: GSE55952 | GEO | 2014/06/01

SECONDARY ACCESSION(S): PRJNA241424

REPOSITORIES: GEO

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Streptococcus mutans UA159 grown in the presence of glucose or maltose pH7 and pH5

Project description:Transcriptional analysis of the effects of sugar source in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159, UA159 grown in glucose or maltose in continuous culture to steady-state pH values of 7 or 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2014-06-01 | E-GEOD-55952 | biostudies-arrayexpress

Streptococcus mutans UA159 vs. fabT mutant strain, pH5 vs. pH7

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Streptococcus mutans UA159 vs. fabM mutant strain (UR117), pH7 vs. pH5/5.5

Project description:Transcriptional analysis of glucose shock vs. steady-state growth in the parent strain and an acid sensitive mutant strain of S. mutans

2015-02-15 | GSE64236 | GEO

Streptococcus mutans UA159 vs. fabT mutant strain, pH5 vs. pH7

Project description:Transcriptional analysis of the regulator FabT in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and MU1591 (M-bM-^HM-^FfabT) grown in continuous culture to steady-state pH values of 7 and 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2012-05-31 | E-GEOD-36960 | biostudies-arrayexpress

Streptococcus mutans UA159 vs. fabM mutant strain (UR117), pH7 vs. pH5/5.5

Project description:Transcriptional analysis of glucose shock vs. steady-state growth in the parent strain and an acid sensitive mutant strain of S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and UR117 (fabM mutant strain) grown in continuous culture to a steady-state pH value of 7. The cultures were exposed to a glucose shock (200mM) and samples were collected upon achieving culture pH value of 5.5. pH control was re-established and cultures were allowed to grow to a steady-state pH value of 5 (for UA159) and 5.5 (for fabM mutant). RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2015-02-15 | E-GEOD-64236 | biostudies-arrayexpress

Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH5

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans

2014-04-01 | GSE56118 | GEO

Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH7

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans

2012-10-04 | GSE38803 | GEO

Streptococcus mutans UA159

Project description:Global transcriptional analysis of S. mutans sugar transporters

| PRJNA99301 | ENA

Streptococcus mutans UA159 with or without 8.4% oxygen vs. nox mutant strain. pH5

Project description:Transcriptional analysis of the effects of oxygen concentraion in S. mutans RNA was extracted from 3 or 4 replicate samples of S. mutans UA159, UA159 grown in 8.4 % oxygen concentration and MU1020 (M-bM-^HM-^Fnox) grown in continuous culture to steady-state pH values of 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

2014-04-01 | E-GEOD-56118 | biostudies-arrayexpress

Transcriptomic profiles of Streptococcus mutans UA159 grown in the presence or absence of mucins or mucin glycans

Project description:We use high-throughput sequencing to profile the response of oral commensal pathogen Streptococcus mutans to mucins protein polymers (human MUC5B mucins) and soluble mucin glycans (human MUC5B glycans and porcine MUC5AC glycans). We find that mucins and their glycans alter the regulation of dozens of S. mutans genes, specifically downregulating competence-associated quorum sensing genes. The transcriptional responses induced by MUC5B mucins, MUC5B glycans, and MUC5AC glycans are highly correlated.

2021-01-01 | GSE163258 | GEO

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