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Gene expression of early craniofacial development through RNA-seq

ABSTRACT: We report gene expression patterns in embryonic mice (E8.5, E9.5, E10.5) through RNA-seq of 13 tissue/stage combinations in order to generage an atlas of early mouse craniofacial gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE55966 | GEO | 2014/03/18

SECONDARY ACCESSION(S): PRJNA241446

REPOSITORIES: GEO

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Gene expression of early craniofacial development through RNA-seq

Project description:We report gene expression patterns in embryonic mice (E8.5, E9.5, E10.5) through RNA-seq of 13 tissue/stage combinations in order to generage an atlas of early mouse craniofacial gene expression. Embryos at E8.5, E9.5, and E10.5 CD1 were collected from CD1 mice, fom multiple micro-regions whose development, differentiation and signaling are responsible for the construction of the mammalian face.

2014-03-18 | E-GEOD-55966 | biostudies-arrayexpress

A gene expression atlas of early craniofacial development

Project description:We present a gene expression atlas of early mouse craniofacial development. Laser capture microdissection (LCM) was used to isolate cells from the principal critical micro-regions, whose development, differentiation and signaling interactions are responsible for the construction of the mammalian face We examined the facial mesenchyme and adjacent neuroepithelium at E8.5, at E9.5 we obtain cells from the facial mesenchyme, olfactory placode/epidermal ectoderm, underlying neuroepithileium, and emerging mandibular and maxillary arches. AT E10.5 we sampled the medial and lateral prominences, olfactory pit, multiple regions of the underlying neuroepithelium the mandibular and maxillary arches, and Rathke's pouch. Mouse emrbyos were harvested at developmental stage E8.5 , E9.5, and E10.5 and cells were captured from microregions responsible for the construction of the mammalian face. RNA was extracted, labelled, and quantified using the Mouse ST-l microarray.

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A gene expression atlas of early craniofacial development

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2014-03-18 | GSE55965 | GEO

ATAC-seq, histone-seq, and RNA-seq of mouse craniofacial tissues [RNA-seq]

Project description:To investigate the role of the transcription factor AP-2 in craniofacial development, we performed: ATAC-seq on E11.5 craniofacial surface ectoderm in control and Tfap2a/Tfap2b ectoderm knock-out embryos; RNA-seq on E10.5 craniofacial prominences from control and Tfap2a/Tfap2b ectoderm knock-out embryos; histone-seq (H3K4me3) in E10.5 and E11.5 wild-type craniofacial surface ectoderm.

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ATAC-seq, histone-seq, and RNA-seq of mouse craniofacial tissues [ChIP-seq]

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Single cell RNA-seq of E8.5 mouse embryonic craniofacial tissues

Project description:The craniofacial region encompassing rhombomere 2 and adjacent putative BA1 together with all more anterior tissues was collected from E8.5 mouse embryos, processed and analyzed by 10X Genomics Chromium scRNA-seq

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Epigenomic Atlas of Early Human Craniofacial Development

Project description:Interactions between putative enhancer region and locations on the same chromosome in mouse E11.5 craniofacial tissue

2017-05-09 | GSE98251 | GEO

The mice uterine gene expression landscape from early to late pregnancy

Project description:To define the vast landscape of mice pregnancy and labor/parturition associated gene expression transitions, wild type FVB mice uterine tissues from the non-pregnant state (NP) and at E6.5, E8.5, E10.5, E15.5 and E17.5 were analysed using gene expression microarray

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Profiling epigenomic modifications, H3K4me3 and H3K27ac, during craniofacial development in a marsupial model, the fat-tailed dunnart (Sminthopsis crassicaudata; Dasyuridae)

Project description:Marsupials display accelerated development of the craniofacial region relative to the differentiation of the CNS when compared to placental mammals. This heterochrony is thought to be due to the functional demands on the oral region for suckling post-birth while in a highly altricial state after a very short gestation period. While cis-regulatory regions are thought to play a significant role in morphological evolution of the mammalian face, the genetic mechanisms involved in craniofacial heterochrony in marsupials remain unclear. To assess this question, we generated ChIP-seq data for H3K4me3 and H3K27ac from fat-tailed dunnart (Sminthopsis crassicaudata; Dasyuridae) craniofacial tissue collected from pouch young on the day of birth and characterised the cis-regulatory landscape during craniofacial development. Using data from the mouse ENCODE consortium we explored the similarities and differences in active regulatory pathways between the dunnart and mouse during early craniofacial development. We found that dunnart promoter- and enhancer- associated peaks overlap to some extent with all the stages (E10.5 – E15.5) in the mouse. However, dunnart genes with peaks were significantly enriched for genes that exhibit linear increases in expression from E10.5 to E15.5 in mice, suggesting a greater similarity with late development. In turn, genes displaying this temporal expression pattern were enriched for Gene Ontology terms related to ossification and skeletal development, processes that underlie development of the cranial muscles and bones of the face. This study provides useful insights into genomic regions associated with cis-regulatory elements regulating craniofacial development in marsupials and their potential role in craniofacial heterochrony

2023-03-15 | GSE188990 | GEO

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