Comparison of D. simulans/D. sechellia third instar male larvae hybrid expression values to pure-species larvae parents

ABSTRACT: Species often produce sterile hybrids early in their evolutionary divergence, and some evidence suggests that hybrid sterility may be associated with deviations or disruptions in gene expression. In support of this idea, many studies have shown that a high proportion of male-biased genes are underexpressed compared to non-sex-biased genes in sterile F1 male hybrids of Drosophila species. In this study, we examined and compared patterns of misexpression in F1 hybrid male third instar larvae of Drosophila simulans and its sibling species, D. sechellia. We analyzed hybrids using custom cDNA arrays we developed from RT-PCRs of spermatogenesis-related transcripts from these species and another sibling species (D. mauritiana). The results from a commercial genome-wide array and custom chip for adults of this species pair, from the custom chip and the genome-wide chip for adults of the D. simulans-D. mauritiana species pair, and from the larvae of the D. simulans-D. mauritiana species pair, are presented separately. Keywords: Comparison of pure-species Drosophila expression to hybrid expression in larvae Overall design: We analyzed 11 arrays: 4 of sim-sec vs. sec, 4 of sim-sec vs. sim, and one each of self-hybridizations (sim vs. sim, sim-sec vs. sim-sec, sec vs. sec).

OTHER RELATED OMICS DATASETS IN: PRJNA96863 PRJNA104367 PRJNA104365 PRJNA104363 PRJNA104371 PRJNA104355 PRJNA104369

INSTRUMENT(S): Drosophila sperm chip v1.0

SUBMITTER: Amanda J. Moehring  

PROVIDER: GSE5598 | GEO | 2006-09-13

SECONDARY ACCESSION(S): PRJNA104363

REPOSITORIES: GEO