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CGH of stages 10B and 13 amplifying follicle cells to determine the effect SuUR has on replication fork progression

ABSTRACT: Comparative genomic hybridization was performed to compare amplificaiton gradients in genomic DNA derived from stage 13 egg chambers of wild-type, suppressor of under-replication (SuUR) mutant and SuUR overexpression, compared with 0-2hr diploid embryo gDNA. Comparative genomic hybridization was performed to compare amplificaiton gradients in genomic DNA derived from stage 10B egg chambers of wild-type, compared with 0-2hr diploid embryo gDNA.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE56054 | GEO | 2014/11/10

SECONDARY ACCESSION(S): PRJNA242311

REPOSITORIES: GEO

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CGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila

Project description:Comparative genomic hybridization was performed to compare levels of gDNA in third instar salivary glands of Drosophila mutants/nulls in the SuUR and orc proteins, compared with 0-2hr diploid embryo gDNA. This illustrates regions of differential replication in the genome.

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Project description:We analyzed gamma-H2Av ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from wild-type (OrR) or suppressor of under-replication (SuUR) mutant Drosophila. Goals were to determine the DNA damage profile relative to underreplicated domains. We analyzed SUUR and Cdc45 ChIP-Seq from hand dissected early (stage 10) and late (stage 12/13) egg chambers from adult Drosophila ovaries. The goals was to determine the localization of SUUR relative to replication forks.

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Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of RNAPolII ChIP from third instar salivary glands relative to control IgG pulldown for two different Drosophila strains

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