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Influence of hexafluoro-2-propanol (HFIP) on lipopolysaccharides-induced inflammation in human microvascular endothelial cells of the lung

ABSTRACT: Hexafluoro-2-propanol (HFIP), a water-soluble primary metabolite of the volatile anesthetic sevoflurane, has recently been shown to have favorable properties, improving overall survival in a chronic model of murine peritonitis. The mechanisms of protection provided by HFIP are currently unknown. We therefore performed a gene expression analysis in an in vitro model of endotoxin-induced endothelial cell injury to gain insight into involved molecular mechanisms. Pathway analysis was subsequently performed using the GeneGo Metacore Framework.

ORGANISM(S): Homo sapiens

PROVIDER: GSE56256 | GEO | 2017/03/14

SECONDARY ACCESSION(S): PRJNA242764

REPOSITORIES: GEO

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Project description:Although sevoflurane is one of the most commonly used inhalational anesthetic agent, the popularity of desflurane is increasing to a similar level. The main beneficial property of desflurane is the relatively fast emergence of the patient from the anesthetic state after halting its supply compared with anesthesia using other anesthetic agents. However, there has been no comprehensive comparison of the effects of these two anesthetic agents on alterations in liver gene expression profiles in animals, including humans, to assess the kinds of biological abnormalities/changes and their levels in the host. Thus, we compared alterations in the global gene expression profiles in the livers of rats subjected to inhalational anesthesia by sevoflurane or desflurane by a next-generation sequencing method.

2024-02-21 | GSE244436 | GEO

Transcription profiling of human atrial tissue from patients undergoing coronary artery bypass graft surgery to determine the effect of anesthesia on gene expression

Project description:Anesthetic gases elicit organ protection in patients undergoing coronary artery bypass graft (CABG) surgery. This study aimed at identifying myocardial transcriptional phenotypes and anesthetic-induced changes in gene expression to predict cardiovascular biomarkers and cardiac function after off-pump CABG. Experiment Overall Design: Patients scheduled for off-pump CABG were randomized into a group with the anesthetic gas sevoflurane (n=10) or the intravenous anesthetic propofol (n=10). Atrial samples were collected at the beginning and end of bypass surgery to determine gene expression profiles.

2008-11-22 | E-GEOD-4386 | biostudies-arrayexpress

sevoflurane exposures on neonatal and adult mice,LC-MSMS

Project description:Sevoflurane is the most commonly used general anesthetic in pediatric surgery, but it has the potential to be neurotoxic. Previous research found that long-term or multiple sevoflurane exposures could cause cognitive deficits in newborn mice but not adult mice, whereas short-term or single inhalations had little effect on cognitive function at both ages. The mechanisms behind these effects, however, are unclear. In the current study, 6- and 60-day-old C57bl mice in the sevoflurane groups were given 3% sevoflurane plus 60% oxygen for three consecutive days, each lasting 2 hours, while those in the control group only got 60% oxygen. The cortex tissues were harvested on the 8th or 62nd day. The tandem mass tags (TMT)pro-based quantitative proteomics combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysi were applied to analyze the influences of multiple sevoflurane anesthesia on the cerebral cortex in mice with various ages. A total of 6247 proteins were measured using the combined quantitative proteomics methods of TMTpro-labeled and LC-MS/MS, 443 of which were associated to the age-dependent neurotoxic mechanism of repeated sevoflurane anesthesia. Our findings would help to further the mechanistic study of age-dependent anesthetic neurotoxicity and contribute to seek for effective protection in the developing brain under general anesthesia.

2022-12-08 | PXD037294 | Pride

Transcription profiling of human cell cultures, MCF7 and SYS5Y, subjected to volatile anesthetic gasses

Project description:Human cell cultures (MCF7 and SY5Y) were subjected to volatile anesthetic gasses in an operation theater and monitored for full genome gene expression characteristics to learn more about the time dependent molecular mechanisms that occur during surgery and to find the implications that anesthetics may have in finding gene expression fingerprints.

2010-10-10 | E-NCMF-32 | biostudies-arrayexpress

Gene expression of brain regions activated by sevoflurane and propofol

Project description:The basic mechanisms of action for general anesthesia are not fully understood. We thought differences in the neural activation sites of different types of anesthetics and differences in gene expression changes in those brain regions may lead to differences in the incidence of side effects. We performed gene expression analysis using microarrays in the identified regions that have been related to the mechanisms of anesthetic action and side effects. Gene expression changes in these brain regions were determined for sevoflurane and propofol to understand the mechanisms that cause differences among anesthetics.

2021-12-22 | GSE192399 | GEO

Propofol and Sevoflurane Anesthesia in Colorectal Cancer Surgery: Incidence of Acute Kidney Injury

Project description:The goal of this observational study is to compare anesthetic modalities (intravenous propofol anesthesia with sevoflurane gas anesthesia) in patients who underwent colorectal cancer resection surgery regarding the outcome of acute kidney injury. The main questions it aims to answer are: * is there a difference in acute kidney injury incidence in the two anesthetic modalities? * is there a difference in plasma creatinine between the two anesthetic modalities? * are there any patient characteristics or intraoperative factors that effect the incidence of acute kidney injury in either anesthetic modality? The study will analyze data from the CAN clinical trial database. (Cancer and Anesthesia: Survival After Radical Surgery - a Comparison Between Propofol or Sevoflurane Anesthesia, NCT01975064)

| 2751857 | ecrin-mdr-crc

m6A RNA methylation profiling in hippocampus of POCD mice induced by sevoflurane

Project description:Elderly patients are apt to cognitive impairment and memory loss after surgical operations. This perioperative cerebral damage named postoperative cognitive dysfunction (POCD) is profoundly affected by anesthesia. N6-methyladenosine (m6A) RNA methylation as a widely-studied epigenetic modification to regulate gene expression, however, is never studied in POCD. Initially, fifty 40-week-old outbred female C57BL/6 mice were conducted Morris water maze test by EthoVision XT working system (Noldus, Netherlands) as manufacturer’s instructions. The escaping latent period of each mouse were recorded. Then Thirty mice were randomly selected and given 2% sevoflurane for 4 h in an automatic anesthetic chamber with size of 24 cm*12 cm*18 cm. After natural resuscitation, POCD and non-POCD mice were picked up based on the dynamics of escaping latent period. The other twenty mice received normal air were treated as negative control. Hippocampus were conducted RIP-seq for investigating m6A RNA methylation.

2021-05-31 | E-MTAB-10510 | biostudies-arrayexpress

RNA sequencing of young mice hippocampus after anesthesia

Project description:We performed RNA sequencing for hippocampus of neonatal mice that were exposed to sevoflurane. We treated a group of neonatal mice with 2.5% sevoflurane for 2 hours on day 6, 7, 8, 9 and treated another group on day 6, 7. On day 36, we collected RNA (both total RNA and polysome-associated RNA) from the hippocampus in the aforementioned two groups and the control group (i.e., without sevoflurane treatment). We prepared the sequencing libraries with the collected RNA and sent them to Illumina sequencing platform.

2023-03-09 | E-MTAB-10202 | biostudies-arrayexpress

Profiling circulating microRNA expression in experimental sepsis by cecal ligation and puncture

Project description:To profile the expression of circulating microRNAs (miRNAs) of mice in experimental sepsis by cecal ligation and puncture (CLP), the whole blood samples were obtained from C57BL/6 mice at 4, 8, and 24 h following CLP for miRNA expression analysis using a miRNA array (The Mouse & Rat miRNA OneArray® v3). Briefly, mice were anesthetized with a combination of ketamine and xylazine as the anesthetic/analgesic agents and a midline abdominal incision was made. The cecum was mobilized, ligated in the middle of cecum below the ileocecal valve, punctured once with a 21 G needle, and a little stool was squeeze out of the cecum to induce polymicrobial peritonitis. The abdominal wall was closed in two layers. Sham-operated mice underwent the same procedure, including opening the peritoneum and exposing the bowel, but without ligation and needle perforation of the cecum.

2015-05-15 | GSE47094 | GEO

Profiling circulating microRNA expression in experimental sepsis by cecal ligation and puncture

2015-05-15 | E-GEOD-47094 | biostudies-arrayexpress

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