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Bromodomain dependence of BRD4-dependent gene expression in mouse fibroblasts

ABSTRACT: To study the role of the bromodomain (BD) in BRD4-dependent gene expression, we compared the function of wild type BRD4 and a mutant BRD4-mBD, which carries a point mutation in each of the two BDs. We first knocked down endogenous BRD4 by shRNA (shBRD4) and then stably reconstituted the cells with shBRD4-resistant YFP-BRD4 (wild type or mBD mutant). Following BRD4 reconstitution, the degree of recovery in gene expression was analyzed by Affymetrix Mouse Exon 1.0 ST array. Wild-type BRD4 restored gene expression significantly and far more efficiently than the mBD mutant.

ORGANISM(S): Mus musculus

PROVIDER: GSE56370 | GEO | 2014/11/10

SECONDARY ACCESSION(S): PRJNA243060

REPOSITORIES: GEO

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BRD4 assists elongation of both coding and enhancer RNAs guided by histone acetylation

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