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MicroRNA expressions in human hepatocarcinoma cells in response to HBV X protein expression

ABSTRACT: MicroRNA expression profiling of human Hep3B and HepG2 HCC cells comparing parental cancer cells with stable HBx-transfectants

ORGANISM(S): Homo sapiens

PROVIDER: GSE56424 | GEO | 2014/04/02

SECONDARY ACCESSION(S): PRJNA243289

REPOSITORIES: GEO

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MicroRNA expressions in human hepatocarcinoma cells in response to HBV X protein expression

Project description:MicroRNA expression profiling of human Hep3B and HepG2 HCC cells comparing parental cancer cells with stable HBx-transfectants In this study,two pairs of control and HBx-overexpressing HCC cells were used to acquire expression profiles of a total of microRNA. miRNA microarray analysis was performed with mirVana miRNA Bioarrays V1 (Ambion) according to the manufacturerM-bM-^@M-^Ys instructions

2014-04-02 | E-GEOD-56424 | biostudies-arrayexpress

Hepatitis B virus X protein contributes to hepatocellular carcinoma via upregulation of KIAA1429 methyltransferase and mRNA m6A hypermethylation of HSPG2/Perlecan [MeRIP-Seq]

Project description:Chronic hepatitis B virus (HBV) remains to be the most common risk factor of hepatocellular carcinoma (HCC). While work has primarily focussed on understanding the direct and indirect mechanisms of Hepatitis B virus X protein (HBx) mediated hepatocarcinogenesis, from genetic and epigenetic perspectives, its influence on RNA modification mediated onset of liver malignancies is less well understood. This study explored the role of HBV-encoded HBx in altering the m6A methylome profile and its implications on the pathogenesis of HCC. We established HBx expressing stable HCC cell lines, Huh7-HBx and HepG2-HBx, and explored the transcriptomic and epitranscriptomic profiles by RNA-seq and MeRIP-seq, respectively. Preliminary results suggest that HBx promotes liver cell proliferation, migration, survival and overall m6A methylation in HCC cells and is involved in modulating the extracellular matrix. We show that HBx mediates liver cell transformation by upregulating KIAA1429 methyltransferase. HBx also drives the expression and hypermethylation of the extracellular matrix protein HSPG2/Perlecan and promotes tumourigenesis. Our findings indicate a potential interaction between KIAA1429 and HSPG2, thus could be novel targets in combating HBx-driven HCC and warrants further investigation.

2023-08-09 | GSE240450 | GEO

Hepatitis B virus X protein contributes to hepatocellular carcinoma via upregulation of KIAA1429 methyltransferase and mRNA m6A hypermethylation of HSPG2/Perlecan [RNA-Seq]

2023-08-09 | GSE240394 | GEO

Proteome of Human hepatocellular carcinoma cell lines

Project description:The proteomic profiling of nine commonly used HCC cell lines Hep3B, HepG2, HepG2.2.15, HUH7, PLC/PRF/5, MHCC97L,MHCC97H,HCCLM3 and HCCLM6

2022-11-11 | PXD036643 | Pride

RNA-seq data for parental and lenvatinib-resistant HCC cells

Project description:RNA-seq data for parental and lenvatinib-resistant HCC (Hep3B, Huh7) cells

2022-03-23 | GSE186191 | GEO

miRNAs expression in HepG2-HBx and HepG2-URG11

Project description:Compare microRNAs expression when HepG2 cells stably expressing HBx protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase Compare microRNAs expression when HepG2 cells stably expressing URG11 protein with HepG2 control cells expressing baterial chloramphenicol acetyltransferase microRNA expression in Cells expressing HBx vs. Cell without HBx microRNA expression in Cells expressing URG11 vs. Cell without URG11

2011-11-23 | E-GEOD-33854 | biostudies-arrayexpress

Wild-type versus HBx-transfected mive

Project description:Hepatitis B virus (HBV) has been clearly recognized as an etiological factor for hepatocellular carcinoma (HCC). HBV encodes the potentially oncogenic HBx protein. We aimed to elucidate the molecular mechanism of HCC caused by HBx and to discover the biomarker related to HCC by HBx. Three experimental groups, 3, 9 and 13 month aged HBx Tg mice and age matched normal wild type B6 mouse which have same background of HBx Tg mice were used to find differentially expressed genes during HCC. Keywords: Genetic modification 3-month-old, 9-month-old, 13-month-old wild type B6 mice vs 3-month-old, 9-month-old, 13-month-old HBx transfected mice; Biological replicates at each timepoint; 9 controls vs 9 HBx-mice

2010-06-19 | E-GEOD-12723 | biostudies-arrayexpress

Global miRNA sequencing upon HDAC inhibition in HCC

Project description:Global miRNA expression upon HDAC inhibition was analyzed using miRNA sequencing in HCC cell lines (HLE, HLF, Huh7, HepG2, Hep3B) and normal liver cell lines (THLE-2, THLE 3)

2022-05-26 | GSE182460 | GEO

sRNA sequencing to compare small non-coding RNA profiles of hepatocellular carcinoma cell lines Huh7, HepG2, and Hep3B against the normal liver cell HL7702

Project description:To evaluate small non-coding dysregulation in HCC cells, we sequenced hepatocellular carcinoma cell lines Huh7, HepG2, and Hep3B and normal liver cell HL7702 to compare their mall non-coding RNA profiles.

2024-01-17 | GSE215349 | GEO

Wild-type versus HBx-transfected mive

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