Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E14.5 mouse cortical neurospheres in response to Fezf2 over-expression

ABSTRACT: RNA Sequencing of E14.5 mouse cortical neurospheres in response to Fezf2 over-expression

ORGANISM(S): Mus musculus

PROVIDER: GSE56446 | GEO | 2014/07/06

SECONDARY ACCESSION(S): PRJNA243346

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

E14.5 mouse cortical neurospheres in response to Fezf2 over-expression

Project description:RNA Sequencing of E14.5 mouse cortical neurospheres in response to Fezf2 over-expression 2 replicates each of GFP-transfected or Fezf2/GFP-transfected E14.5 mouse cortical neurospheres. Paired-end sequencing 101bp.

2014-07-06 | E-GEOD-56446 | biostudies-arrayexpress

Fezf2 overexpression in murine cortical progenitors in vivo

Project description:Corticospinal motor neurons (CSMN) are one specialized class of cortical excitatory neurons, which connect layer Vb of the cortex to the spinal cord. a master transcription factor –Forebrain expressed zinc finger 2 (Fezf2) – has been identified that is necessary for the fate specification of CSMN. Fezf2 alone can cell-autonomously instruct the acquisition of CSMN-specific features when expressed in diverse, permissive cellular contexts, in vivo. In order to understand the molecular logic underlying the acquisition of CSMN traits upon Fezf2 expression, we compared the in vivo gene expression of FACS-purified cortical progenitors that ectopically expressed Fezf2 to control progenitors. We used in utero electroporation to deliver Fezf2GFP or CtrlGFP expression vectors to neocortical progenitors at E14.5, when they primarily generate CPN of the upper layers. Overexpression of Fezf2 in these progenitors is sufficient to instruct a fate-switch resulting in the generation of CSMN and other subtypes of corticofugal projection neurons. Fezf2GFP- and CtrlGFP -electroporated progenitors were FACS-purified at 24 and 48 hours after surgery and acutely profiled by microarrays.

2014-07-06 | E-GEOD-56451 | biostudies-arrayexpress

Fezf2 overexpression in murine cortical progenitors in vivo

2014-07-06 | GSE56451 | GEO

Gene expression changes in differentiated cortical neurospheres pre-exposed to ethanol

Project description:Cerebral cortical-derived neurosphere cultures were exposed to ethanol to model heavy alcohol exposure during the period of cortical neurogenesis Keywords: Compared differentiated neurospheres pre-exposed to ethanol to untreated differentiated neurospheres

2006-09-30 | GSE5152 | GEO

ATAC-seq for Pantr2 knockout cortical neurospheres

Project description:We used assay for transposase accessible chromatin with sequencing (ATAC-seq) to discover changes in chromatin accessibility in Pantr2 KO cortical neurospheres

2022-08-02 | GSE210135 | GEO

Mus musculus

Project description:E14.5 mouse cortical neurospheres in response to Fezf2 over-expression

| PRJNA243346 | ENA

Expression data from Ankrd11Yod/+ and WT embryonic cortical neurospheres

Project description:Ankrd11 is a potential chromatin regulator implicated in neural development and autism spectrum disorder (ASD) with no known function in the brain. Here, we show that knockdown of Ankrd11 in developing murine or human cortical neural precursors caused decreased proliferation, reduced neurogenesis, and aberrant neuronal positioning. Similar cellular phenotypes and aberrant ASD-like behaviors were observed in Yoda mice carrying a point mutation in the Ankrd11 HDAC-binding domain. Consistent with a role for Ankrd11 in histone acetylation, Ankrd11 was associated with chromatin, colocalized with HDAC3, and expression and histone acetylation of Ankrd11 target genes were altered in Yoda neural precursors. Moreover, the Ankrd11 knockdown-mediated decrease in precursor proliferation was rescued by inhibiting histone acetyltransferase activity or expressing HDAC3. Thus, Ankrd11 is a crucial epigenetic regulator of neural development that controls histone acetylation and gene expression, thereby providing a likely explanation for its association with cognitive dysfunction and ASD. We used microarrays to compare the gene expression profile in embryonic neurospheres prepared from neocortices of WT and Ankrd11Yod/+ mice E14.5 cortical secondary neurosheres 5 days post-passage were collected and total RNA extracted. cDNA was hybridized on Affymetrix Mouse Gene 2.0 ST Array and gene expression was analyzed using Parterk software. In total, 6 Ankrd11Yod/+ and 5 WT embryos were used.

2014-11-15 | E-GEOD-63303 | biostudies-arrayexpress

RNA-seq of postnatal day 0 (P0) wild-type, Fezf2-/- cortices, and Fezf2-/-;Fezf2-EnR cortices

Project description:The goal of the study was to compare gene expression of P0 wild-type, P0 Fezf2-/- cortices, and Fezf2-/-; Fezf2-EnR cortices. Total RNAs were isolated from P0 cortices dissected from wild-type (n=3), Fezf2-/- mice (n=4), and Fezf2-/-; Fezf2-EnR cortices (n=2), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA PolyA library preparation guide.The libaries were pair-end sequenced (150nt per end). Differentially expressed genes were identified by DESEQ.

2021-06-11 | GSE160202 | GEO

Single Cell sequencing of E14.5, E15.5 and E14.5 Kir electroporated cortical neurons.

Project description:Single Cell sequencing of E14.5, E15.5 and E14.5 Kir electroporated cortical neurons.

| PRJNA475585 | ENA

Transcription profiling of CD133/Prominin positive neurospheres

Project description:To identify expression of hematopoetic stem cell antigens in cerebral cortical derived neurospheres Keywords: Compared CD133 positive neurospheres to control GFP-positive neurospheres

2006-10-01 | GSE5171 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data