Transcriptomics,Genomics

Dataset Information

29

Effect of obesity on liver immune response to inflammatory stimuli in a diet-obesity model of zebrafish (Danio rerio)


ABSTRACT: We used a Diet-Induced-Obesity approach using the zebrafish (Danio rerio) based on overfeeding to analyze the liver transcriptomic modulation in the disease and to determine how the obesity affects the immune response against an acute inflammatory stimulus as the lipopolysacharide (LPS). Overfed zebrafish were obese and showed signs of steatosis in their liver. Furthermore, the gene modulation profile resembled to that observed in humans. After the inflammatory stimulus, there was a clear differential immune response in normal and overfed fish. In normal fish, the response to LPS reported a typical host defensive reaction comparable to the one occurring in stimulated mammals whereas there was not any significantly modulated gene when comparing the expression of liver from LPS-stimulated with non-stimulated obese zebrafish. Overall design: Healthy fish were fed two times a day, one with Artemia and the other with standard fish chow while obese fish were fed fish chow two more times a day during 8 months. 10 μg of pure LPS (Sigma) or PBS was intraperitoneally injected and livers were sampled and pooled at 3 hours post injection.

INSTRUMENT(S): Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version)

SUBMITTER: Gabriel Forn-Cuní 

PROVIDER: GSE56478 | GEO | 2015-03-23

SECONDARY ACCESSION(S): PRJNA243389

REPOSITORIES: GEO

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Publications

Liver immune responses to inflammatory stimuli in a diet-induced obesity model of zebrafish.

Forn-Cuní Gabriel G   Varela Monica M   Fernández-Rodríguez Conrado M CM   Figueras Antonio A   Novoa Beatriz B  

The Journal of endocrinology 20141104 2


Obesity- and metabolic syndrome-related diseases are becoming important medical challenges for the western world. Non-alcoholic fatty liver disease (NAFLD) is a manifestation of these altered conditions in the liver, and inflammation appears to be a factor that is tightly connected to its evolution. In this study, we used a diet-induced obesity approach in zebrafish (Danio rerio) based on overfeeding to analyze liver transcriptomic modulation in the disease and to determine how obesity affects t  ...[more]

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