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Peripheral blood mononuclear cell gene expression pre-/post- oral lipid challenge

ABSTRACT: Acute metabolic challenges provide insight on how the body responds to metabolic stress. In this study, we assessed transcriptomic response in peripheral blood mononuclear cells (PBMC) to an acute lipid challenge.

ORGANISM(S): Homo sapiens

PROVIDER: GSE56608 | GEO | 2015/09/01

SECONDARY ACCESSION(S): PRJNA244073

REPOSITORIES: GEO

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Peripheral blood mononuclear cell gene expression pre-/post- oral glucose challenge

Project description:Acute metabolic challenges provide insight on how the body responds to metabolic stress. In this study, we assessed transcriptomic response in peripheral blood mononuclear cells (PBMC) to an acute glucose challenge.

2015-09-01 | GSE56607 | GEO

Gene expression profiling of PBMCs in dairy cows pre- and post-partum

Project description:The transition to lactation challenges dairy cows metabolically. Immune dysfunction and infectious disease risk is the hallmark of this transition period. Transcriptome data of PBMC shows differentially expressed pathways postpartum. Metabolically stressed cows show upregulation of innate immune pathways and inflammation.

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Peripheral blood mononuclear cell gene expression pre-/post- acute metabolic challenge

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-09-01 | GSE56609 | GEO

The Impact of Blood-Processing Time on the Proteome of Human Peripheral Blood Mononuclear Cells

Project description:Human peripheral blood mononuclear cells (PBMC) are key to several diagnostics assays and basic science research. Blood pre-analytical variations that occur before obtaining the PBMC fraction can have a significant impact on the results of the assays, including viability, composition, integrity, and gene expression changes of immune cells. With this as motivation, we performed a quantitative shotgun proteomics analysis using Isobaric Tag for Relative and Absolute Quantitation (iTRAQ 8plex) labeling to compare PBMC obtained from fresh versus 24h-stored blood at room temperature. We identified 3,195 proteins, of which 245 were differentially abundant. Our results revealed enriched pathways in the fresh-PBMC samples related to exocytosis, localization, vesicle-mediated transport, cell activation, and secretion. In contrast, pathways related to exocytosis, neutrophil degranulation and activation, granulocyte activation, leukocyte degranulation, and myeloid leukocyte activation involved in immune response were enriched in the 24h-PBMC samples, which may indicate probable granulocyte contamination and activation due to blood storage time and temperature. Examples of upregulated proteins in the 24h-PBMC sample are: CAMP, S100A8, LTA4H, RASAL3, and S100A6, which are involved in an adaptive immune system and antimicrobial activity, proinflammatory mediation, aminopeptidase activities, and naïve T cells survival. Moreover, examples of downregulated proteins are: NDUFA5, TAGLN2, H3C1, TUBA8, and CCT2 that are related to the cytoskeleton, cell junction, mitochondrial respiratory chain. In conclusion, blood-processing time directly impacts the proteomic profile of human PBMC.

2020-12-14 | PXD021367 | Pride

Transcriptome profiling of peripheral blood mononuclear cell subsets

Project description:Transcriptome profiling was conducted on 2 replicate samples of total peripheral blood mononuclear cells (PBMC) and isolated PBMC subsets.

2020-04-14 | GSE101489 | GEO

Effects of oral-glucose load on the gene expression of peripheral blood mono-nuclear cells in Asian-Indian men.

Project description:The objective of this study was to perform a global, non-targeted gene expression analysis by microarray, to understand the immune cell gene regulation at fasting and in response to oral glucose load and how this regulation is different in Asian-Indian men with normal glucose tolerance (NGT) and pre-diabetes (PD). Through observing real-time gene expression changes, this study highlights 1. the importance of acute metabolic challenges like oral glucose load in regulating the immune cell gene expression and function. 2. How insulin resistance and pre-diabetes causes severe perturbation of baseline gene expression and thus alters the response to oral glucose load as well.

2021-07-06 | GSE153837 | GEO

RNA-seq of human peripheral blood mononuclear cells following oral cholera vaccination

Project description:In this study we performed RNA-seq to identify human transcriptomic signatures associated with antibody responses to an oral cholera vaccine. We isolated total RNA from peripheral blood mononuclear cells of vaccinated volunteers who following two priming oral immunisations, had either strong or no antibody responses to the antigen components of the cholera vaccine. We performed a longitudinal and comparative PBMC transcriptional assessment of high vs low antibody vaccine responders at day 0, 19 and 44 post vaccination. We also identified unique and overlapping genes of low and high vaccine responders 0, 19 and 44 post vaccination. Due to General Data Protection Regulation (GDPR) access to human sequenced raw data is restricted and will be made available upon request through https://doi.org/10.17044/scilifelab.12213434

2021-03-23 | E-MTAB-9069 | biostudies-arrayexpress

Gene expression profiling using cDNA microarray of HMGB1fl/fl and HMGB1ΔHep liver samples from mice subjected to a 12 week-HFD regimen or a Fasting/Refeeding challenge

Project description:To unveil any gene expression difference induced by hepatocyte specific-Hmgb1 gene deletion in mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge. Challenges known to generate a robust activation of hepatic lipogenesis and liver steatosis.

2022-03-31 | E-MTAB-11541 | biostudies-arrayexpress

Proteome dataset of peripheral blood mononuclear cells in postpartum dairy cows supplemented with different sources of omega-3 fatty acids

Project description:This article contains raw and processed data related to research published by Kra et al. [1]. There is a scarce knowledge on the proteome of peripheral blood mononuclear cells (PBMC) during the transition period in dairy cows. In human research, proteomics PBMC is used in order to gain insight into inflammatory diseases and syndromes. Dietary fats, and specifically omega-3 (n-3) FA, can moderate the immune fluctuation caused by parturition through improvements of the immune function [2]. Therefore, this study aim was to characterize the changes that may occur in proteome of PBMC during transition, as influenced by different n-3 FA supplementation. Proteomics data of PBMC was obtained from postpartum dairy cows supplemented peripartum with either encapsulated saturated fat (CTL), encapsulated flaxseed oil that is enriched with ALA (α-linolenic acid; FLX) or encapsulated fish oil that is enriched with EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid; FO).The analysis was done by liquid chromatography-mass spectrometry from PBMCs protein extraction. The cells were collected from six cows per treatment during the 1st week postpartum. Quantification of differential abundance between groups was done using MS1 intensity based label-free. Label-free quantitative shotgun proteomics was used for characterization. This novel dataset of proteomics data from PBMC contains 3807 proteins; 44, 42 and 65 were differently abundant (P ≤ 0.05 and FC ± 1.5), in FLX vs. CTL, FO vs. CTL and FLX vs. FO, respectively; these findings are discussed in our recent research article [1]. The present dataset of PBMC proteome adds new information regarding the effects of n-3 FA on the immune system, while providing reference for PBMC proteome in postpartum dairy cows.

2021-11-17 | PXD029683 | Pride

Plasma fatty acid levels and gene expression related to lipid metabolism in peripheral blood mononuclear cells

Project description:Solid evidence indicates that intake of marine n-3 fatty acids lower serum triglycerides, and that replacing saturated fatty acids (SFA) with polyunsaturated fatty acids (PUFA) reduces plasma total cholesterol and LDL-cholesterol. The molecular mechanisms underlying these health beneficial effects are however not completely elucidated. The aim of this study was to investigate the expression of genes related to lipid metabolism in peripheral blood mononuclear cells (PBMC) depending on the plasma levels of n-6 and n-3 fatty acids and the SFA to PUFA ratio.

2019-03-08 | GSE111567 | GEO

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