Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA profile of dG9a-depleted early embryo in Drosophila melanogaster

ABSTRACT: Immunocytochemical studies revealed that dG9a moves into nucleus after cycle 8 and appears to regulate gene expression by di-methylating H3K9 from cycle 8 to cycle 11. To determine which genes are regulated by dG9a during cycles 8-11, we examined mRNA levels by performing RNA-sequence analysis using early embryos (0-2 h after egg laying) of dG9a null mutant and wild type as a control

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE56665 | GEO | 2015/08/01

SECONDARY ACCESSION(S): PRJNA244257

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

mRNA profile of dG9a-depleted early embryo in Drosophila melanogaster

Project description:Immunocytochemical studies revealed that dG9a moves into nucleus after cycle 8 and appears to regulate gene expression by di-methylating H3K9 from cycle 8 to cycle 11. To determine which genes are regulated by dG9a during cycles 8-11, we examined mRNA levels by performing RNA-sequence analysis using early embryos (0-2 h after egg laying) of dG9a null mutant and wild type as a control mRNA profiles of about 0-2h-old embryos of wild type (CantonS) and dG9a-depleted (dG9aRG5) strain

2015-08-01 | E-GEOD-56665 | biostudies-arrayexpress

D. melanogaster eye-antennal imaginal discs developmental transcriptome

Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).

2018-01-08 | GSE94915 | GEO

Transcription profiling of Drosophila melanogaster homozygous mutant embryos in a timecourse of embryogenesis

Project description:Expression profiling of Drosophila Lmd (lame-duck) homozygous mutant embryos in a timecourse of embryogenesis. Five one-hour timepoints were assayed (5-11 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.

2010-06-01 | E-TABM-894 | biostudies-arrayexpress

CUTnTag against Beaf-32, CP190 and CTCF in Drosophila melanogaster wild-type and insulator-depleted embryos at stage NC14

Project description:CUTnTag against Beaf-32, CP190 and CTCF was performed in wild-type and insulator-depleted embryos collected between 2h10 and 2h40 after egg laying (corresponding to the stage NC14). Spike-ins from D. virilis were used in each reaction. For each sample, 50k nuclei from each species were mixed together at the start of the protocol. For each protein, CUTnTag was done in biological duplicates. Sequencing was performed on Illumina NextSeq 500.

2022-12-17 | E-MTAB-11993 | biostudies-arrayexpress

Differential circular RNAs expression in ovary during oviposition in honey bees

Project description:The present study is the first study to identify the involvement of circRNAs in the ovary activation and oviposition regulation processes in honey-bee queens.CircRNAs expresion profiles were examined in ovaries of virgin queens, egg-laying queens, egg-laying inhibited queens and egg-laying recovery queens.

2018-06-07 | GSE103860 | GEO

Tissue-specific DNase-seq during Drosophila melanogaster embryogenesis

Project description:Chromatin accessibility mapping by DNase-seq on FACS-isolated cell populations during Drosophila melanogaster embryogenesis (6-8 hrs after egg-laying)

2018-01-29 | E-MTAB-5999 | biostudies-arrayexpress

Integration of lncRNA-miRNA-mRNA reveals novel insights into reproductive regulation in honey bees

Project description:The present study is the first study to identify the involvement of mRNA, lncRNAs and miRNA in the ovary activation and oviposition regulation processes in honey-bee queens. mRNA, lncRNA and miRNA expresion profiles were examined in ovaries of virgin queens, egg-laying queens, egg-laying inhibited queens and egg-laying recovery queens.

2017-09-14 | GSE93028 | GEO

Drosophila melanogaster whole embryo RNA-Seq experiments during embryogenesis

Project description:Sequencing of mRNAs of 22 wild type strains from the Drosophila Genetic reference Panel (DGRP) at embryonic time point 10-12 hrs after egg laying was performed. ~100 embryos were pooled for each biological sample prior to library preparation.

2017-01-10 | E-MTAB-4723 | biostudies-arrayexpress

Gut microbiota-driven regulation of queen bee ovarian metabolism

Project description:With the global prevalence of Varroa mites, more and more beekeepers resort to confining the queen bee in a queen cage to control mite infestation or to breed superior and robust queen bees. However, the impact of such practices on the queen bee remains largely unknown. Therefore, we subjected the queen bees to a 21-d egg-laying restriction treatment (from the egg stage to the emergence of adult worker bees) and analyzed the queen bees' ovarian metabolites and gut microbiota after 21 d, aiming to assess the queen bee's quality and assist beekeepers in better hive management. Our findings revealed a significant reduction in the relative expression levels of Vg and Hex110 genes in the ovaries of egg-laying-restricted queen bees compared to unrestricted egg-laying queens. The diversity of gut microbiota in the queen bee exhibited a notable decrease, accompanied by corresponding changes in the core bacterial species of the microbial community. Following egg-laying restriction, the activity of the queen bee's ovaries decreased, while the metabolism of glycerophospholipids remained or stored more lipid molecules, awaiting environmental changes for the queen bee to resume egg-laying promptly. Furthermore, we observed that Bombella in the queen bee's gut may regulate the queen's ovarian metabolism through tryptophan metabolism. These findings provide novel insights into the interplay among queen egg-laying, gut microbiota, and ovarian metabolism.

2023-09-11 | MTBLS7576 | MetaboLights

Drosophila melanogaster 4C-Seq experiments in whole embro and mesoderm cells during embryogenesis

Project description:Chromosome conformation capture (4C-Seq) in Drosophila Twist-H2B embryos (carrying nuclear tag specifically in the mesoderm) during embryogenesis was performed, anchoring on 107 different viewpoints. Two timepoints (3-4hrs and 6-8hrs after egg laying) and two tissue context (whole embryo and mesoderm) were assayed. Two independent collections were performed at each timepoint.

2014-06-26 | E-MTAB-2180 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data