Single-Cell Whole-Genome Bisulfite Sequencing for Analysis of Epigenetic Heterogeneity

ABSTRACT: We report a single-cell whole-genome bisulfite sequencing method (scBS-Seq) capable of accurately measuring DNA methylation at up to 36% of CpGs. We observed that ESCs grown in serum/LIF or 2i/LIF both display epigenetic heterogeneity, with “2i-like” cells present in serum cultures. In silico integration of 12 individual MII oocytes datasets  recapitulates the whole DNA methylome, making scBS-Seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE56879 | GEO | 2014/07/18

SECONDARY ACCESSION(S): PRJNA244869

REPOSITORIES: GEO