Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide transcriptome analysis of neuron-enriched DRG cultures exposed to Wnt3a

ABSTRACT: To test the potential of canonical Wnt signalling to modulate nociception via transcriptional regulation in dorsal root ganglion (DRG) neurons, we performed a genome-wide transcriptome analysis of neuron-enriched DRG cultures treated with Wnt3a or vehicle for 6 hours.

ORGANISM(S): Mus musculus

PROVIDER: GSE56880 | GEO | 2014/07/07

SECONDARY ACCESSION(S): PRJNA244879

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide transcriptome analysis of neuron-enriched DRG cultures exposed to Wnt3a

Project description:To test the potential of canonical Wnt signalling to modulate nociception via transcriptional regulation in dorsal root ganglion (DRG) neurons, we performed a genome-wide transcriptome analysis of neuron-enriched DRG cultures treated with Wnt3a or vehicle for 6 hours. Total RNA obtained from neuron-enriched mouse DRG culture subjected to Wnt3a treatment for 6 hours was compared to a matched control (vehicle-treated) DRG culture.

2014-07-07 | E-GEOD-56880 | biostudies-arrayexpress

Genome-wide RNA expression analysis of rat dorsal root ganglion tissue after local absorbable collagen sponge implantation and BMP-2 exposure

Project description:Analysis of whole dorsal root ganglion tissue expression signatures in responses to absorbable collagen sponge implantation with and without BMP-2 exposure. The hypothesis is that inflammatory macrophage-associated responses to ACS implantation contributes to nociception in the DRG and spinal nerve.

2017-01-27 | GSE77384 | GEO

Whole-genome gene expression profiling of F11 dorsal root ganglion (DRG)-like cells stimulated with forskolin

Project description:We generated whole-genome gene expression profiles of F11 dorsal root ganglion (DRG)-like neuroblastoma cells stimulated with the adenylate cyclase activator forskolin for 0, 2, 4, 24 and 48 hours.

2010-07-01 | GSE22631 | GEO

WT vs Hypomethylated DRG Gene Expression

Project description:Although DNA methylation plays a critical role in the development and function of mammalian central nervous system (CNS), its role in peripheral neurons has not been elucidated. To address this issue, we produced conditional knockout mice (CKO) specifically deleting the gene for maintenance DNA methyltransferase 1 (Dnmt1) during the development of neural crest cells. Despite global hypomethylation in the embryonic dorsal root ganglion (DRG) of the CKO mice, the number of sensory neurons was relatively unaffected. However, expression of many genes required for sensory neuron development was altered in embryonic mutant DRG, including down-regulation of Runx1 and TrkA genes as well as up-regulation of Id1 and Dtx1, two negative regulators for neurogenesis. Accompanied with the downregulation of an NGF receptor TrkA, the peripheral axonal projection and the branching of sensory neurons were impaired. Furthermore, the expression of the neuropeptide Galanin and several vanilloid receptors such as TrpV1 and TrpM8 were not detected in the DRG of the CKO mice during late embryonic and neonatal stages, suggesting that DNA methylation regulates the differentiation program for a subset of nociceptive sensory neurons. Taken together, our findings suggest that through transcriptional regulation of key developmental genes in sensory neurons, DNA methylation play a key role in the control of the axonal projection and fate specification of peripheral sensory neurons. We compared gene expression patterns in Wildtype and DNA methylation deficient (Wnt1-cre; Dnmt1 mutant) mouse dorsal cortex. We performed 4 replicates using different each individual mouse strain. The Sample GSM565172 table is the average log ratio for the 4 replicatesArrays were performed.

2011-12-01 | E-GEOD-23530 | biostudies-arrayexpress

Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity

Project description:We performed a data independent acquisition (DIA) -based quantitative proteomics strategy to investigate the global proteome alteration in the dorsal root ganglion (DRG) tissues from mice injected with oxaliplatin for different periods.

2022-07-11 | PXD031322 | Pride

microarray_submission

Project description:We evalueted gene expression in dorsal root ganglion (DRG) over-expressing NRG1 typeIII ICD (Intra Cellular Domain). DRG were infected with a lentivirus expressing ICD and compared to not infected DRG or DRG infected with a lentivirus expressing EGFP.

2014-12-14 | E-MTAB-2131 | biostudies-arrayexpress

Proteomics on Nav1.8-positive nociceptors of mouse DRG

Project description:Nociceptors play an essential role in both acute pain and chronic pain conditions. In this study, we examined the proteome of mouse dorsal root ganglia and compared NaV1.8Cre+/-; ROSA26-flox-stop-flox-DTA (Diphtheria toxin fragment A) mutant mice (NaV1.8Cre-DTA), in which NaV1.8-positive neurons (mainly nociceptors) in dorsal root ganglia (DRG) were ablated, with respective littermate wildtype controls.

2022-10-03 | PXD034447 | Pride

TNT model of neuropathic pain

Project description:In this experiment we compare the effect of tibial nerve transection on gene expression within the dorsal root ganglion (DRG) of rats.

2014-08-01 | E-MTAB-2260 | biostudies-arrayexpress

The Nicotinic alpha6 Subunit Gene Determines Variability in Chronic Pain Sensitivity and Nicotine Anti- allodynia via Cross-inhibition of P2X2/3 Receptors.

Project description:We used microarray-based expression genomics in 25 inbred mouse strains to identify dorsal root ganglion (DRG)-expressed genetic contributors to mechanical allodynia a prominent symptom of chronic pain. Expression genetics identifies a role for the Chrna6 (alpha 6-nicotinic receptor) gene in pain in mice and humans. Dorsal root ganglion tissue across multiple inbred mouse strains, both male and female

2015-02-18 | E-GEOD-65997 | biostudies-arrayexpress

Conditioning lesion rat DRG time course

Project description:To identify potential regeneration-associated genes, rat dorsal root ganglia (DRG) were lesioned and then sampled at 4, 8, 12, 24, 48, 148, or 296 hrs A total of 23 samples were examined in 8 time points with 2-3 replicates each. For each array, lesioned and uninjured DRG samples were run in a two-color experiment. ----------------------------------------------------- submitters of this record cannot locate the raw data

2015-07-06 | E-GEOD-69466 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data