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Analysis of RNA from primary hepatocytes from hepatocyte-specific SRSF1 knockout mice vs. WT

ABSTRACT: RNA from primary hepatocyte cultures from three 3-month-old SRSF1HKO mice compared to RNA from three 3-month-old WT mice for changes in exon utilization and gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE57020 | GEO | 2015/05/01

SECONDARY ACCESSION(S): PRJNA245221

REPOSITORIES: GEO

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Analysis of RNA from primary hepatocytes from hepatocyte-specific SRSF1 knockout mice vs. WT

Project description:RNA from primary hepatocyte cultures from three 3-month-old SRSF1HKO mice compared to RNA from three 3-month-old WT mice for changes in exon utilization and gene expression. SRSF1 KO vs. WT, three replicates each.

2015-05-01 | E-GEOD-57020 | biostudies-arrayexpress

Analysis of RNA from liver tumors in SRSF3 hepatocyte knockout mice

Project description:RNA from three hepatocellular carcinomas from 24-month-old SRSF3HKO mice compared to RNA from non-tumorous liver from 24-month-old SRSF3HKO mice for changes in exon utilization and gene expression.

2015-05-01 | GSE57019 | GEO

Analysis of RNA from liver tumors in SRSF3 hepatocyte knockout mice

2015-05-01 | E-GEOD-57019 | biostudies-arrayexpress

Analysis of liver RNA from SRSF3 hepatocyte knockout mice versus wild-type littermates

Project description:Livers from three SRSF3HKO mice and 3 wild-tyope littermates were used to analyze changes in exon utilization and gene expression.

2012-12-01 | GSE35686 | GEO

Analysis of liver RNA from SRSF3 hepatocyte knockout mice versus wild-type littermates

Project description:Livers from three SRSF3HKO mice and 3 wild-tyope littermates were used to analyze changes in exon utilization and gene expression. two genotypes, three replicates each

2012-12-01 | E-GEOD-35686 | biostudies-arrayexpress

Priming hepatocytes into G2 phase reverses age-related regenerative impairments in livers of elderly mice

Project description:Frozen liver tissue from 24 month old C57BL/6 mice; control or each of the other three treatment groups taken pre and 36 hour post hepatectomy were used for analysis (n=2 per group). Mice were administrated intraperitoneally with one of the following agents every day for 7 days and 10 minutes before hepatectomy or hepatocyte isolation: 0.5 ml of saline, 500 umol alpha-aminobutyric acid or 500 umol gamma-aminobutyric acid in 0.5ml of saline. Mice in control group received no pre-administrations.

2014-08-29 | E-MTAB-2810 | biostudies-arrayexpress

Hepatocyte cells

Project description:Hepatocyte Cell lysate analyzed via three different activation methods

2019-03-07 | PXD008604 | JPOST Repository

Minimal expression of dysferlin prevents development of muscular dystrophy in dysferlin exon 40a knockout mice (second dataset)

Project description:Dysferlin is an essential muscle membrane repair protein and autosomal recessive mutations in its gene result in progressive muscular dystrophies collectively called dysferlinopathies. We previously showed that calpain-mediated cleavage within dysferlin exon 40a releases a 72kDa C-terminal minidysferlin recruited to injured sarcolemma. In the current study, we hypothesised that knocking out exon 40a will lead to defective membrane repair and development of muscular dystrophy over time. To address this hypothesis, we created three exon 40a knockout (40aKO) mouse lines with dysferlin protein levels ranging from ~80% of wildtype (WT) in 40aKO-3, ~50% in 40aKO-2 and ~10-20% in 40aKO-1 mice. Histopathological analysis of skeletal muscles harvested from all 12-month-old 40aKO mice showed little evidence of dystrophic features seen in dysferlin-null BLAJ mice. Lipidomics analysis on 18wk old quadriceps showed that all 40aKO lines had a similar lipidomic profile to WT and distinct from BLAJs. The proteomic profile of 40aKO lines was intermediate between that of WT and BLAJs. Laser membrane damage assays showed normal membrane repair capacity in all three 40aKO lines. These results collectively indicate that ~10-20% of dysferlin protein expression is sufficient for maintenance of the lipidome and membrane repair capacity, and crucially prevents development of muscular dystrophy.

2023-03-11 | PXD035918 | Pride

Minimal expression of dysferlin prevents development of muscular dystrophy in dysferlin exon 40a knockout mice

2023-03-11 | PXD035393 | Pride

Gene expression profiling of normal mouse hepatocyte, premalignant hepatocytes and fully malignant HCC

Project description:Gene expression was analyzed and compared of normal mouse hepatocyte, premalignant hepatocytes and fully malignant HCC cells. The results provide valuable information about the gene expression alterations during the chronic process of liver cancer development. HCC in age-matched male mice were induced by DEN injection. Normal mouse hepatocyte, premalignant hepatocytes and fully malignant HCC were freshly isolated and RNA extracted.

2013-10-11 | E-GEOD-50431 | biostudies-arrayexpress

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