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Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays (GPL14921)

ABSTRACT: We investigated whether mouse serum autoantibody binding patterns on random-sequence peptide microarrays (immunosignaturing) can be used for diagnosing and predicting the onset of lupus and its central nervous system (CNS) manifestations. Submitter states "We have no processed data to submit. We have no gpr files to submit."

ORGANISM(S): synthetic construct Mus musculus

PROVIDER: GSE57388 | GEO | 2014/05/08

SECONDARY ACCESSION(S): PRJNA246380

REPOSITORIES: GEO

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Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays (GPL14921)

Project description:We investigated whether mouse serum autoantibody binding patterns on random-sequence peptide microarrays (immunosignaturing) can be used for diagnosing and predicting the onset of lupus and its central nervous system (CNS) manifestations. Submitter states "We have no processed data to submit. We have no gpr files to submit." To identify possible predictive and diagnostic peptides of lupus and CNS-lupus, we carried out two studies and selected peptides in common across both studies. In the first study we tested 3-6 MRL/lpr, MRL/mp and C3H/HeJ mice at four months of age. For study two we tested 9-10 MRL/lpr and MRL/mp at 1.5 and 4 months of age. In both studies the mice sera were diluted 1/500 and analyzed using microarray peptides from platform GPL14921. We ran each sample in triplicate. The MRL/lpr and MRL/mp are the autoimmune strains and the C3H/HeJ is the control strain.

2014-05-08 | E-GEOD-57388 | biostudies-arrayexpress

Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays (GPL17600)

2014-05-08 | GSE57389 | GEO

Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays (GPL17600)

Project description:In addition to determining possible diagnostic and predictive peptides of lupus and CNS-lupus, we also used our microarray technology along with the Guitope computer program to determine possible natural protein match to five monoclonal autoantibodies that were created using one of the autoimmune MRL/lpr mouse. Submitter states "We have no processed data to submit. We have no gpr files to submit." Microarray analysis was performed on five monoclonal brain-reactive autoantibodies (F9, G10, G4, D1 and D9) that were created from one autoimmune mouse with altered behavior. These samples were tested in duplicated along with a control sample on the GPL17600 platform.

2014-05-08 | E-GEOD-57389 | biostudies-arrayexpress