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Medicago abi3 mutant analysis

ABSTRACT: Comparison of transcriptomes of WT and abi3 mutant lines at 32 and 40 days after pollination

ORGANISM(S): Medicago truncatula

PROVIDER: GSE57457 | GEO | 2014/05/09

SECONDARY ACCESSION(S): PRJNA246518

REPOSITORIES: GEO

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Medicago abi3 mutant analysis

Project description:Comparison of transcriptomes of WT and abi3 mutant lines at 32 and 40 days after pollination Transcriptome of Medicago WT lines (3 replicates) compared with Medicago abi3 mutant lines (NF6003) using Medicago_v1 NimbleGen chip

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Overexpression of MtABI3 splicing forms by ectopically expressing cDNA sequences in transgenic Medicago truncatula hairy roots generated from an abi3 mutant background (mutant NF6003)

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated specifically by different splicing forms in the model legume Medicago truncatula, Medicago hairy roots obtained from an abi3 mutant background (NF6003) were generated using Agrobacterium rhizogenes transformed with the three cDNA sequence of the ABI3 gene splicing forms of Medicago. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control.

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Identification of target genes of ABI3 [Agilent 44k]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify genes regulated by ABI3 we performed array based transcriptome analysis of Dexamethasone inducible ABI3 transgenic seedlings. ABI3 mostly in concert with abscisic acid (ABA) was found to activate genes specifically expressed during the maturation phase of seed development.

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ABI3 Network Walking

Project description:Deciphering gene regulatory networks (GRNs) is a key for understanding gene expression regulations in living systems. Here, we describe the investigation of the ABSCISIC ACID INSENSITIVE 3 (ABI3) plant transcription factor GRN vicinity by a technique called Network Walking. The method involves transient transformation of protoplasts and inducible nuclear re-localization of transcription factors along with transcriptomic analysis. This genome-wide approach allowed the de novo recovery of i) direct and indirect ABI3 target genes, ii) cis-binding site preference, and iii) biological processes regulated by this canonical abscisic acid response factor. This work improves our knowledge of ABI3 action by inferring network motifs (such as Feed Forwar Loops) under its influence. The novel high-throughput-oriented technique will help accelerate GRN systems investigations in plants, as well as in other organisms. This work studies ABI3 direct and indirect targets by a technique named Network Walking. Root/protoplasts were treated with or without dexamethasone (DEX) and cycloheximide (CHX). 3 reps each.

2013-01-01 | E-GEOD-33344 | biostudies-arrayexpress

Identification of target genes of ABI3

Project description:This SuperSeries is composed of the following subset Series: GSE33951: Identification of target genes of ABI3 [Agilent 44k] GSE34033: Identification of target genes of ABI3 [Agilent 2x244k] GSE34041: Identification of target genes of ABI3 [SAP] Refer to individual Series

2012-05-31 | E-GEOD-34043 | biostudies-arrayexpress

Comparison of genes differentially expressed between Medicago R108 and abi3 mutants (NF3185 and NF6003) during different stages of seed development (16, 24, 32 and 40 DAP)

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated specifically by ABI3 in the model legume Medicago R108 during seed development, using the Medicago NimbleGen chip, a transcriptomic analysis was performed between abi3 mutants (NF3185 and NF6003) and R108 wild-type at 4 stages of seed development.

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ABI3 TARGET approach

2013-01-01 | GSE33344 | GEO

Identification of target genes of ABI3 [SAP]

Project description:ABI3 is a key regulator of seed development in Arabidopsis and other plants.To identify DNA fragments (promoters) bound by ABI3 we performed array based ChIP analysis.

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