Transcriptomics,Genomics

Dataset Information

58

6KR-EGFR expressing MCF7 following EGF or HRG stimulation


ABSTRACT: EGF and HRG, growth factor ligands for EGFR and ErbB3/4 receptor, induce transient and sustained ERK activity associated with cellular proliferation and differentiation of MCF-7 cells, respectively. To rigorously analyze the effect of ERK signal duration for mRNA expression dynamics and its relationship with cell determination, we modified the EGF-triggered ERK signal duration by changing the EGFR activation dynamics by impairing the ubiquitination and degradation process. Mutation of the six lysine residues (6KR; K692, K713, K730, K843, K905 and K946) of the EGFR responsible for ubiquitin conjugation has shown sustained phosphorylation of the receptor (Huang et al, 2006; Goh et al, 2010). Therefore we constructed the MCF-7 cell lines that stably express 6KR EGFR (6KR), and analyzed signaling and mRNA expression dynamics in response to EGF and HRG. Overall design: 6KR-EGFR (6KR) expressing MCF7 human breast cancer cells were stimulated by the growth hormone (epidermal growth factor (EGF) or heregulin (HRG)). Control was set as non-treated cells.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Mariko Okada-Hatakeyama  

PROVIDER: GSE57547 | GEO | 2014-12-01

SECONDARY ACCESSION(S): PRJNA246684

REPOSITORIES: GEO

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Publications

Feedforward regulation of mRNA stability by prolonged extracellular signal-regulated kinase activity.

Nagashima Takeshi T   Inoue Norihiko N   Yumoto Noriko N   Saeki Yuko Y   Magi Shigeyuki S   Volinsky Natalia N   Sorkin Alexander A   Kholodenko Boris N BN   Okada-Hatakeyama Mariko M  

The FEBS journal 20150108 4


Extracellular signal-regulated kinase (ERK) plays a central role in signal transduction networks and cell fate decisions. Sustained ERK activation induces cell differentiation, whereas transient ERK results in the proliferation of several types of cells. Sustained ERK activity stabilizes the proteins of early-response gene products. However, the effect of ERK activity duration on mRNA stability is unknown. We analyzed the quantitative relationship between the duration of four ERK activity kineti  ...[more]

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