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Analysis of the evolutionary adaptation of an AraC-like global regulatory protein in the murine enteric pathogen Citrobacter rodentium

ABSTRACT: An experiment to test the equivalence of the Citrobacter rodentium RegA and E.Coli SMS 3-5 homologue

ORGANISM(S): Citrobacter rodentium

PROVIDER: GSE57689 | GEO | 2015/02/19

SECONDARY ACCESSION(S): PRJNA247724

REPOSITORIES: GEO

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Analysis of the evolutionary adaptation of an AraC-like global regulatory protein in the murine enteric pathogen Citrobacter rodentium

Project description:An experiment to test the equivalence of the Citrobacter rodentium RegA and E.Coli SMS 3-5 homologue 8 Conditions (4 Genotypes by 2 treatments) in duplicate. Reference design without dye swaps.

2015-02-19 | E-GEOD-57689 | biostudies-arrayexpress

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[E-MTAB-502] Citrobacter rodentium transcriptomics (Regulation)

Project description:Whole genome trancription study of Citrobacter rodentium grown in rich media. Publication Title: Citrobacter rodentium is an Unstable Pathogen Showing Evidence of Significant Genomic Flux Publication Author List: Nicola K. Petty, Theresa Feltwell, Derek Pickard, Simon Clare, Ana L. Toribio, Maria Fookes, Kevin Roberts, Rita Monson, Satheesh Nair, Robert A. Kingsley, Richard Bulgin, Siouxsie Wiles, David Goulding, Craig Corton, Nicola Lennard, David Harris, David Willey, Richard Rance, Lu Yu, Jyoti S. Choudhary, Carol Churcher, Michael A. Quail, Julian Parkhill, Gad Frankel, Gordon Dougan, George P.C. Salmond, Nicholas R. Thomson ArrayExpress Release Date: 2011-02-12 Person Roles: investigator Person Last Name: Thomson Person First Name: Nicholas Person Mid Initials: Person Email: nrt@sanger.ac.uk Person Phone: Person Address: Wellcome Trust Genome Campus, Hinxton, Cambridge, UK Person Affiliation: Wellcome Trust Sanger Institute Person Roles: submitter Person Last Name: Service Person First Name: Submission Person Mid Initials: Person Email: datahose@sanger.ac.uk Person Phone: Person Address: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom Person Affiliation: Wellcome Trust Sanger Institute

2011-07-14 | GSE30610 | GEO

Citrobacter rodentium

Project description:[E-MTAB-502] Citrobacter rodentium transcriptomics (Regulation)

| PRJNA144515 | ENA

Polysaccharides from Sargassum fusiforme protect mice against Citrobacter rodentium infection through intestinal microbiota-drived microRNA-92a-3p

Project description:To explore the regulatory mechanism of intestinal flora in Citrobacter rodentium -induced intestinal infection by transcriptome analysis at miRNA molecular level.

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Control of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA

Project description:Identification of the targets of RegA with and without bicarbonate stimulation by comparing RegA knockout to multicopy RegA transgenics. RegA is an AraC like transcription factor identified in a mutational screen for virulence genes in Citrobacter rodentium, an attaching and effacing pathogen that causes transmissible colonic hyperplasia in mice. This experiment compares the RegA null strain with a multicopy plasmid rescue of this null strain in the presence and absence of bicarbonate with the aim of identifying pathogenesis related genes related to the early and late stages of attachment and effacement. Keywords: genetic modification, transcription factor, induction A strain of Citrobacter rodentium with a knockout of RegA was compared to the same strain rescued with a multicopy plasmid containing the wildtype RegA gene. These strains were analyzed with and without bicarbonate in an unconnected two factor design with dye balanced biological replicates.

2010-05-19 | E-GEOD-12876 | biostudies-arrayexpress

Citrobacter rodentium transcriptomics (Regulation)

Project description:Citrobacter rodentium transcriptomics (Regulation)

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