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Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

ABSTRACT: In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE57786 | GEO | 2014/05/20

SECONDARY ACCESSION(S): PRJNA248191

REPOSITORIES: GEO

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Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.

2014-05-20 | E-GEOD-57786 | biostudies-arrayexpress

Expression profiling of SMMC-7721 cells: stably express lncRNA-ATB vs pcDNA3.1

Project description:lncRNA-ATB induced transcriptional changes in SMMC-7721

2014-02-08 | GSE54798 | GEO

Expression profiling of SMMC-7721 cells: stably express lncRNA-PRAL vs control

Project description:lncRNA-PRAL induced transcriptional changes in SMMC-7721

2017-11-24 | GSE63599 | GEO

Expression profiling of SMMC-7721 cells: stably express lncRNA-ATB vs pcDNA3.1

Project description:lncRNA-ATB induced transcriptional changes in SMMC-7721 Two-condition experiment, control (pcDNA3.1) vs lncRNA-ATB expression (pcDNA3.1-ATB). Each has 3 biological repeats.

2014-02-08 | E-GEOD-54798 | biostudies-arrayexpress

Expression data from SMMC-7721 cells

Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells The liver cancer cell line SMMC-7721 transfected with either the control or POH1 siRNAs were subjected to a genome wide transcription profile analysis through Human U133 Puls 2.0 (Affymetrix) microarray

2015-01-23 | E-GEOD-65210 | biostudies-arrayexpress

Expression data from SMMC-7721 cells

Project description:We performed a genome wide transcription profile analysis to determine the expression alterations between control and the POH1 siRNAs transfected SMMC-7721 cells

2015-01-23 | GSE65210 | GEO

Long noncoding RNA expression profiles in sub-lethal heat treated hepatoma carcinoma cells. (SMMC-7721 cells: sub-lethal heat treated vs. untreated)

Project description:Transcriptional profiling of SMMC-7721 cells comparing control untreated with sub-lethal heat treated cells (50°C for 10 min). Differentially expressed lncRNA and mRNA were measured with an Agilent Human lncRNA+mRNA Array V4.0 (4 × 180 K format) containing 41,000 lncRNAs and 34,000 mRNAs. Goal was to determine the effects of sub-lethal heat treatment on global hepatoma carcinoma cells gene expression.

2017-05-27 | GSE99351 | GEO

The effects of ETFA knockdown on the gene expression in SMMC-7721 human hepatoma cells

Project description:SMMC-7721 single clones stably expressing control shRNA or ETFA shRNAs 1# and 2# were generated and subjected to microarray.

2020-09-22 | GSE104125 | GEO

Transcription profiling by array of human hepatocarcinoma SMMC-7721 cells treated with a new compound IMB5043 against untreated controls

Project description:The whole human genome microarray was used for analysis of the differentially expressed genes in SMMC-7721 cells when exposed to 1 μM IMB5043 treatment for 24 h.

2018-01-22 | E-MTAB-6359 | biostudies-arrayexpress

Gene expression signatures for miR-26a over-expression and negative control in SMMC-7721 cells

Project description:To further development of our gene expression approach to microRNA-26a over-expression, we have employed whole mRNA microarray expression profiling as a discovery platform. SMMC-7721 cells at 70–80% confluence in 6-well plates were transfected using Lipofectamine 2000 (Invitrogen). Negative control mimics or miR-26a mimics (100nM) were transfected in each well. Cell extracts were prepared 48 h after transfection, total RNA was checked for a RIN number to inspect RNA integration by Affymetrix PrimeView™ Human Gene Expression Arrays. We used microarrays to detail the global programme of gene expression underlying miR-26a over-expression and identified distinct classes of up-regulated or down-regulated genes during this process.

2018-12-24 | GSE108501 | GEO

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