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Genome-Wide Small RNA Profiling of a Soybean Seed Compartment-Cotyledon Stage

ABSTRACT: We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation.

ORGANISM(S): Glycine max

PROVIDER: GSE57883 | GEO | 2014/05/22

SECONDARY ACCESSION(S): PRJNA248316

REPOSITORIES: GEO

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Genome-Wide Small RNA Profiling of a Soybean Seed Compartment-Cotyledon Stage

Project description:We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation. Eight subregions of the Cotyledon stage of the soybean seeds were analyzed. Two biological replicates were collected for each subregions.

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Project description:We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for “making a soybean seed.”

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